beta 2-AR Antibody (I3D6) recommended for detection of β2-AR of human origin by WB, IP, IF and FCM

β2-AR Antibody (I3D6): sc-81578

β2-AR Antibody (I3D6) is rated 3.0 out of 5 by 1.
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Datasheets

    • β2-AR Antibody (I3D6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified, intact β2-adrenergic receptor of human origin
    • recommended for detection of β2-AR of human origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See β2-AR (E-3): sc-271322 for β2-AR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can β2-AR (I3D6): sc-81578 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, β2-AR (I3D6): sc-81578 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the 2-AR protein via Western Blot analysis. -SCBT QC
Date published: 2014-09-11
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