beta 2-AR Antibody (R11E1) recommended for detection of β2-AR of human origin by WB, IP, IF, IHC(P) and FCM

β2-AR Antibody (R11E1): sc-81577

β2-AR Antibody (R11E1) is rated 3.8 out of 5 by 8.
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Datasheets

    • β2-AR Antibody (R11E1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified, intact β2-adrenergic receptor of human origin
    • beta 2-AR Antibody (R11E1) is recommended for detection of β2-AR of human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-beta 2-AR Antibody (R11E1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β2-AR (R11E1): sc-81577.

I would like to know if the B2-AR (R11E1): sc-81577 antibody contains the entire heavy chain + kappa light chain. I need the Fc region for proximity ligation assays. Thank you.

Asked by: KNMM
Thank you for your question. All our monoclonal antibodies are complete antibodies. They all contain two heavy chains and two light chains. We specify which type of light chain the antibody contains (kappa or lambda) for each antibody. This antibody has kappa light chains.
Answered by: Tech Service
Date published: 2019-06-24

Hi, What is the binding region of this antibody? Does it bind to extracellular part of the receptor? Thank you

Asked by: Kazzz
Thank you for your question. This antibody is specific for Lamin A/C. It was raised against purified, intact β2-AR of human origin, the exact epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-30

貴社のβ2-AR Antibody (R11E1)でリンパ球を染め,FMC解析をする場合、生細胞そのままで染色できますか?

Asked by: Yuetsu
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can β2-AR (R11E1): sc-81577 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, β2-AR (R11E1): sc-81577 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Does not IP endogeous or overexpressibg B2AR This antibody did not immunoprecipitate the receptor In HEK293 cells that transiently overexpress it or in others that are know to endogenously express it to high levels.
Date published: 2018-09-17
Rated 5 out of 5 by from Good detection I received this antibody as a free sample to test it in A204 cells
Date published: 2018-03-23
Rated 5 out of 5 by from Worked great for WB I obtained a sample of sc-81577 B2-AR (R11E1) [Lot#G1117] to test on human myoblasts transfected with a non-silencing control siRNA or siRNAs specific to ADRB2, the gene that encodes the B2-AR. Immunodetection via western blotting produced a clear B2-AR-specific band. In contrast, detection of the same samples using sc-271322 B2-AR (E-3) worked much less robustly.
Date published: 2017-08-31
Rated 5 out of 5 by from Excellent for IHC! This antibody is great for IHC on paraffin embedded tissue sections, strong and clean staining.
Date published: 2017-01-26
Rated 4 out of 5 by from Publishable data obtained by WB and co Publishable data obtained by WB and co-IP using human HEK293 cells. -SCBT Publication Review
Date published: 2015-05-12
Rated 4 out of 5 by from Paraformaldehyde fixed rabbit NPE cells analyzed Paraformaldehyde fixed rabbit NPE cells analyzed with product sc-81577 in IF. -SCBT Publication Review
Date published: 2015-04-22
Rated 3 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the 2-AR protein via Western Blot analysis. -SCBT QC
Date published: 2015-04-11
Rated 3 out of 5 by from Produced nice immunoperoxidase cytoplasmic Produced nice immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-01-23
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