beta 2-AR Antibody (E-3) recommended for detection of β2-AR of human origin by WB, IP, IF, IHC(P) and ELISA

β2-AR Antibody (E-3): sc-271322

β2-AR Antibody (E-3) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-β2-AR Antibody (E-3) is a mouse monoclonal IgG2b (kappa light chain) β2-AR antibody provided at 200 µg/ml
    • raised against amino acids 338-413 of β2-AR of human origin
    • beta 2-AR Antibody (E-3) is recommended for detection of β2-AR of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-beta 2-AR Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β2-AR (E-3): sc-271322.

I would like to know the quantity of product. I see the concentration (200ug/ml), but not the volume. Thanks.

Asked by: Timmy
Thank you for your question. Each vial contains 200 µg IgG2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-04-05

Can β2-AR (E-3): sc-271322 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, β2-AR (E-3): sc-271322 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2020-06-23
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Rated 1 out of 5 by from Did not perform well for WB I obtained a sample of sc-271322 B2-AR (E-3) [Lot#K2216] to test on human myoblasts transfected with a non-silencing control siRNA or siRNAs specific to ADRB2, the gene that encodes the B2-AR. Immunodetection via western blotting did not produce a clear B2-AR-specific band. In contrast, detection of the same samples using sc-81577 B2-AR (R11E1) worked much more robustly.
Date published: 2017-08-31
Rated 5 out of 5 by from correct band WB analysis of human liver tissue and got correct band at 1:800 dilution.
Date published: 2017-02-25
Rated 5 out of 5 by from Works Well We used β2-AR antibody from the first day of our experiment, we bought two antibodies but different batches this time, the quality is stable.
Date published: 2017-02-07
Rated 5 out of 5 by from Satisfactory Western blot data of 2 Satisfactory Western blot data of 2-AR expression in non-transfected and human 2-AR transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-17
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