β-TrCP Antibody (C-6): sc-390629

β-TrCP Antibody (C-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects β-TrCP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). β-TrCP (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. β-TrCP protein, also known as E3RSIkB or FWD1, plays a crucial role in the ubiquitin-proteasome pathway, functioning as a substrate recognition subunit of ubiquitin ligases. β-TrCP protein is essential for the regulation of various cellular processes, including the degradation of key regulatory proteins such as CD4, IkB, and β-catenin, which are involved in cell signaling and proliferation. β-TrCP-mediated ubiquitination of these proteins is vital for maintaining cellular homeostasis and preventing aberrant signaling that could lead to diseases such as cancer. β-TrCP′s interaction with other proteins, including HOS (homologous to Slimb), highlights β-TrCP′s importance in the complex network of protein degradation and regulation within the cell. Understanding β-TrCP function and interactions is critical for elucidating β-TrCP′s role in various biological processes and potential as a therapeutic target.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

β-TrCP Antibody (C-6) References:

1. Ubiquitin-dependent degradation of IkappaBalpha is mediated by a ubiquitin ligase Skp1/Cul 1/F-box protein FWD1.  |  Hatakeyama, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 3859-63. PMID: 10097128
2. HOS, a human homolog of Slimb, forms an SCF complex with Skp1 and Cullin1 and targets the phosphorylation-dependent degradation of IkappaB and beta-catenin.  |  Fuchs, SY., et al. 1999. Oncogene. 18: 2039-46. PMID: 10321728
3. A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP).  |  Zhao, B., et al. 2010. Genes Dev. 24: 72-85. PMID: 20048001
4. Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop.  |  Mo, Y., et al. 2022. Mol Cancer. 21: 192. PMID: 36199071
5. SDCBP promotes pancreatic cancer progression by preventing YAP1 from β-TrCP-mediated proteasomal degradation.  |  Liu, J., et al. 2023. Gut. 72: 1722-1737. PMID: 36828627
6. All-Trans Retinoic Acid Promotes a Tumor Suppressive OTUD6B-β-TrCP-SNAIL Axis in Esophageal Squamous Cell Carcinoma and Enhances Immunotherapy.  |  Li, L., et al. 2023. Adv Sci (Weinh). 10: e2207458. PMID: 37038094
7. The novel β-TrCP protein isoform hidden in circular RNA confers trastuzumab resistance in HER2-positive breast cancer.  |  Wang, S., et al. 2023. Redox Biol. 67: 102896. PMID: 37783059
8. Specific targeting of the NRF2/β-TrCP axis promotes beneficial effects in NASH.  |  Fernández-Ginés, R., et al. 2024. Redox Biol. 69: 103027. PMID: 38184999
9. Inhibition of MST1 ameliorates neuronal apoptosis via GSK3β/β-TrCP/NRF2 pathway in spinal cord injury accompanied by diabetes.  |  Huang, W., et al. 2024. Redox Biol. 71: 103104. PMID: 38430683
10. A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif.  |  Margottin, F., et al. 1998. Mol Cell. 1: 565-74. PMID: 9660940
11. Ubiquitination and degradation of the substrate recognition subunits of SCF ubiquitin-protein ligases.  |  Zhou, P. and Howley, PM. 1998. Mol Cell. 2: 571-80. PMID: 9844630
12. Identification of the receptor component of the IkappaBalpha-ubiquitin ligase.  |  Yaron, A., et al. 1998. Nature. 396: 590-4. PMID: 9859996

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-TrCP Antibody (C-6) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-β-TrCP Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-TrCP Antibody (C-6) Specifications

Alternate Product Name: Beta TrCP

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 26-110

Epitope Target: β-TrCP isoforms 1 and 2

Epitope Species: human

Product Reactivity/Detection: β-TrCP isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for β-TrCP Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred β-TrCP/HOS antibody is β-TrCP/HOS Antibody (F-10): sc-166492.