Anti-beta-catenin Antibody (E-5) recommended for detection of β-catenin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-β-catenin Antibody (E-5): sc-7963

Anti-β-catenin Antibody (E-5) is rated 4.8 out of 5 by 101.
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Datasheets

    • Anti-β-catenin Antibody (E-5) is a mouse monoclonal IgG1 κ β-catenin antibody, cited in 773 publications, provided at 200 µg/ml
    • raised against amino acids 680-781 mapping at the C-terminus of β-catenin of human origin
    • Anti-beta-catenin Antibody (E-5) is recommended for detection of β-catenin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-beta-catenin Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-catenin (E-5): sc-7963.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for β-catenin Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with β-catenin Antibody (E-5) (see ordering information below).

What is the suitable antigen retrieval method for this antibody?

Asked by: DV03
Thank you for your question. You can find our recommended protocol with antigen retrieval methods under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2021-04-28

How much is the volume of "β-catenin Antibody (E-5) sc-7963" ?

Asked by: Farideh1
Thank you for your question. All of our normal antibodies have a total volume of 1mL.
Answered by: Tech Service 1
Date published: 2021-02-02

请问,二抗的比例范围是多少?

Asked by: 湖医学子
感谢您的提问。二抗的稀释范围是和您具体的实验方法相关的,产品主页可获取说明书,说明书中有详细的针对不同实验方法的稀释比例范围。 Thank you for your inquiry. The dilution range depend on what application used. You can get more detailed dilution information on its datasheet.
Answered by: Technical Support 8
Date published: 2018-12-21

I need to detect this protein in porcine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary for Western blot?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-14

这款产品测的是磷酸化和非磷酸化的β-catenin的总和吗?有哪一款产品可以直接检测非磷酸化的β-catenin吗?

Asked by: 醫醫123
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Rated 5 out of 5 by from 轻易就能获得漂亮的结果 无论是做IHC还是WB都能获得满意的结果,抗体物美价廉,能够很好的满足实验求,少走弯路,希望以后会有更多好的产品!
Date published: 2021-03-15
Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 4 out of 5 by from The antibody works well in immunofluorescence The antibody performs in a good way in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA
Date published: 2020-11-10
Rated 5 out of 5 by from VERY GOOD AND STABLE FOR IF We have brought this antibody twice. It works well for both IF, IP and WB.
Date published: 2020-09-11
Rated 4 out of 5 by from NIce antibody We bought it two months ago and used it for western blotting. It worked great!
Date published: 2020-01-21
Rated 5 out of 5 by from Great Immunofluorescence IF Works very robustly on mouse organoids at 1:100 dilution
Date published: 2019-12-01
Rated 4 out of 5 by from シグナルよし 293T細胞を使用してウェスタンで検出できました。そこまで強いシグナルではありませんが、変なバックもでないので、問題ありません。
Date published: 2019-11-11
Rated 5 out of 5 by from ottimo Utilizzato 1:200 su 3 linee cellulari di neuroblastoma; molto specifico e pulito; individua la banda attesa di 90 kDa (molto forte) + altre inferiori (lievissime)
Date published: 2019-10-22
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