β-casein Antibody (H-4): sc-166530

β-casein Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects β-casein in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). β-casein Antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. β-casein plays a vital role in the nutritional development of newborn mammals, as β-casein is one of the primary proteins found in mammalian milk, particularly in human milk where β- and k-caseins are the only caseins present. The unique structure of β-casein, characterized by highly phosphorylated sites, is crucial for calcium-binding capacity, which enhances the digestibility of casein micelles in colostrum, thereby supporting the neonate′s early growth and development. The β-casein gene (Bca) is intricately regulated by a combination of transcription factors, including Stat5 and C/EBPβ, which respond to lactogenic hormones such as prolactin, insulin, and hydrocortisone in mammary epithelial cells. Additionally, β-casein expression is negatively regulated by NFκB during pregnancy, highlighting the complex interplay of signaling pathways that govern β-casein production. Anti-β-casein antibody (H-4) is an essential tool for researchers studying milk protein expression and its implications in neonatal nutrition and development.

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β-casein Antibody (H-4) References:

1. Substitution of casein by beta-casein or of whey protein isolate by alpha-lactalbumin does not affect mineral balance in growing rats.  |  Van Dael, P., et al. 2005. J Nutr. 135: 1438-43. PMID: 15930449
2. Effect of beta-casein, kappa-casein and beta-lactoglobulin genotypes on concentration of milk protein variants.  |  Hallén, E., et al. 2008. J Anim Breed Genet. 125: 119-29. PMID: 18363977
3. Interaction between β-casein and whey proteins as a function of pH and salt concentration.  |  Kehoe, JJ. and Foegeding, EA. 2011. J Agric Food Chem. 59: 349-55. PMID: 21133408
4. A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk.  |  Song, F., et al. 2014. Food Chem. 158: 445-8. PMID: 24731368
5. Identification of a NFκB inhibitory peptide from tryptic β-casein hydrolysate.  |  Malinowski, J., et al. 2014. Food Chem. 165: 129-33. PMID: 25038658
6. The Influence of Ca2+ and Zn2+ on the Amyloid Fibril Formation by β-Casein.  |  Wang, J., et al. 2020. Protein Pept Lett. 27: 915-922. PMID: 32186269
7. Changes in Metabolites from Bovine Milk with β-Casein Variants Revealed by Metabolomics.  |  Lv, Z., et al. 2020. Animals (Basel). 10: PMID: 32486279
8. Interaction between Curcumin and β-Casein: Multi-Spectroscopic and Molecular Dynamics Simulation Methods.  |  Zhao, R., et al. 2021. Molecules. 26: PMID: 34443680
9. The effect of arginine on inhibiting amyloid fibril derived from β-casein and the binding studies with multi-spectroscopic techniques.  |  Wang, J., et al. 2022. Spectrochim Acta A Mol Biomol Spectrosc. 282: 121681. PMID: 35917615
10. Gastric Emptying of New-World Milk Containing A1 and A2 Β-Casein Is More Rapid as Compared to Milk Containing Only A2 Β-Casein in Lactose Maldigesters: A Randomized, Cross-Over Trial Using Magnetic Resonance Imaging.  |  Ramakrishnan, M., et al. 2023. Nutrients. 15: PMID: 36839159

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-casein Antibody (H-4) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-β-casein Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-casein Antibody (H-4) Specifications

Alternate Product Name: β-casein

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 101-115

Epitope Target: β-casein

Epitope Species: mouse

Product Reactivity/Detection: β-casein

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for β-casein Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred β-casein antibody is β-casein Antibody (F20.14): sc-53189.