Bek/FGFR2 Antibody (C-8): sc-6930

Bek Antibody (C-8) is a mouse monoclonal IgG2b kappa light chain antibody that targets the Fibroblast Growth Factor Receptor 2 (FGFR2), also known as Bek, a critical receptor tyrosine kinase involved in regulating cellular processes such as proliferation, differentiation, and angiogenesis through fibroblast growth factor (FGF) signaling pathways. Located on the cell surface, FGFR2 binds to FGF ligands, initiating a cascade of intracellular signaling events that are essential for tissue development, repair, and maintenance. The structure of FGFR2 includes an extracellular ligand-binding domain, a single transmembrane helix, and an intracellular tyrosine kinase domain, which are pivotal for its role in signal transduction and interaction with downstream signaling molecules like FRS2 and PLCγ. FGFR2 undergoes post-translational modifications, including glycosylation, which are crucial for its proper folding, stability, and functional activation, thereby enhancing its ability to interact with specific intracellular partners. FGFR2 monoclonal antibody (C-8) specifically recognizes an epitope mapping between amino acids 805-821 at the C-terminus of FGFR2, a region conserved between human and mouse sequences, ensuring reliable detection across species such as mouse, rat, and human. FGFR2 monoclonal antibody (C-8) is versatile in its applications, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Additionally, FGFR2 monoclonal antibody (C-8) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, catering to diverse experimental requirements. By enabling precise detection and analysis of FGFR2, FGFR2 monoclonal antibody (C-8) is an invaluable tool for research into growth factor signaling mechanisms and their roles in developmental biology and disease pathology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bek/FGFR2 Antibody (C-8) References:

1. Deletion of the carboxyl-terminal exons of K-sam/FGFR2 by short homology-mediated recombination, generating preferential expression of specific messenger RNAs.  |  Ueda, T., et al. 1999. Cancer Res. 59: 6080-6. PMID: 10626794
2. Negative autoregulation of fibroblast growth factor receptor 2 expression characterizing cranial development in cases of Apert (P253R mutation) and Pfeiffer (C278F mutation) syndromes and suggesting a basis for differences in their cranial phenotypes.  |  Britto, JA., et al. 2001. J Neurosurg. 95: 660-73. PMID: 11596961
3. Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.  |  Mansukhani, A., et al. 1992. Proc Natl Acad Sci U S A. 89: 3305-9. PMID: 1373495
4. Promoter region of the murine fibroblast growth factor receptor 2 (bek/KGFR) gene.  |  Avivi, A., et al. 1992. Oncogene. 7: 1957-62. PMID: 1408137
5. Development of Isospora belli in Hct-8, Hep-2, human fibroblast, BEK and Vero culture cells.  |  Siripanth, C., et al. 2004. Southeast Asian J Trop Med Public Health. 35: 796-800. PMID: 15916071
6. Cloning and expression of two distinct high-affinity receptors cross-reacting with acidic and basic fibroblast growth factors.  |  Dionne, CA., et al. 1990. EMBO J. 9: 2685-92. PMID: 1697263
7. Recent developments in the cell biology of basic fibroblast growth factor.  |  Rifkin, DB. and Moscatelli, D. 1989. J Cell Biol. 109: 1-6. PMID: 2545723
8. Effects of N-glycosylation on the biochemical properties of recombinant bEKL expressed in Pichia pastoris.  |  Wang, Z., et al. 2018. Enzyme Microb Technol. 114: 40-47. PMID: 29685352
9. A soluble form of K-sam/FGFR2 protein in the culture medium of human gastric cancer cells.  |  Kishi, T., et al. 1994. Biochem Biophys Res Commun. 202: 1387-94. PMID: 8060318
10. Control of BEK and K-SAM splice sites in alternative splicing of the fibroblast growth factor receptor 2 pre-mRNA.  |  Gilbert, E., et al. 1993. Mol Cell Biol. 13: 5461-8. PMID: 8355693
11. Expression of fibroblast growth factor receptors flg and bek during hepatic ontogenesis and regeneration in the rat.  |  Hu, Z., et al. 1995. Cell Growth Differ. 6: 1019-25. PMID: 8547216
12. Expression of FGFR2 BEK and K-SAM mRNA variants in normal and malignant human breast.  |  Luqmani, YA., et al. 1996. Eur J Cancer. 32A: 518-24. PMID: 8814701

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bek/FGFR2 Antibody (C-8) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-Bek/FGFR2 Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bek/FGFR2 Antibody (C-8) Specifications

Alternate Product Name: FGFR2

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 805-821

Epitope Target: Bek

Epitope Species: human

Product Reactivity/Detection: Bek

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for Bek/FGFR2 Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin