BCKDK Antibody (F-10): sc-374424
- BCKDK Antibody (F-10) is a mouse monoclonal IgG1 κ BCKDK antibody provided at 200 µg/ml
- raised against amino acids 1-280 mapping at the N-terminus of BCKDK of human origin
- BCKDK Antibody (F-10) is recommended for detection of BCKDK of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BCKDK Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BCKDK Antibody (F-10) for WB applications. These reagents are now offered in bundles with BCKDK Antibody (F-10) (see ordering information below).
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SEE ALSO...
BCKDK Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects BCKDK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-BCKDK antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BCKDK protein, also known as branched chain ketoacid dehydrogenase kinase, is a 412 amino acid mitochondrial matrix protein that functions as a monomer and features a histidine kinase domain. BCKDK protein is crucial for regulating the activity of the branched-chain alpha-ketoacid dehydrogenase (BCKD) complex, which is essential in the catabolic pathways of branched-chain amino acids (BCAAs) such as valine, leucine, and isoleucine. BCKDK catalyzes the ATP-dependent phosphorylation and inactivation of the BCKD complex, thereby controlling the irreversible oxidative decarboxylation of BCAAs. BCKDK regulation of BCKD activity is vital for maintaining metabolic homeostasis, as dysregulation can lead to metabolic disorders. Understanding BCKDK function and role in BCAA metabolism is important for developing therapeutic strategies for conditions associated with impaired amino acid catabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BCKDK Antibody (F-10) References:
- Structure of rat BCKD kinase: nucleotide-induced domain communication in a mitochondrial protein kinase. | Machius, M., et al. 2001. Proc Natl Acad Sci U S A. 98: 11218-23. PMID: 11562470
- Solution structure and dynamics of the lipoic acid-bearing domain of human mitochondrial branched-chain alpha-keto acid dehydrogenase complex. | Chang, CF., et al. 2002. J Biol Chem. 277: 15865-73. PMID: 11839747
- Branched-chain amino acid catabolism: unique segregation of pathway enzymes in organ systems and peripheral nerves. | Sweatt, AJ., et al. 2004. Am J Physiol Endocrinol Metab. 286: E64-76. PMID: 12965870
- Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases. | Popov, KM., et al. 1992. J Biol Chem. 267: 13127-30. PMID: 1377677
- Molecular mechanism for regulation of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase complex by phosphorylation. | Wynn, RM., et al. 2004. Structure. 12: 2185-96. PMID: 15576032
- Branched-chain amino acids: enzyme and substrate regulation. | Brosnan, JT. and Brosnan, ME. 2006. J Nutr. 136: 207S-11S. PMID: 16365084
- Mitochondrial alpha-ketoacid dehydrogenase kinases: a new family of protein kinases. | Popov, KM., et al. 1997. Adv Second Messenger Phosphoprotein Res. 31: 105-11. PMID: 9344245
- A molecular model of human branched-chain amino acid metabolism. | Suryawan, A., et al. 1998. Am J Clin Nutr. 68: 72-81. PMID: 9665099
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCKDK Antibody (F-10) | sc-374424 | 200 µg/ml | $316.00 | |||
BCKDK Antibody (F-10): m-IgG Fc BP-HRP Bundle | sc-529742 | 200 µg Ab; 10 µg BP | $354.00 | |||
BCKDK Antibody (F-10): m-IgGκ BP-HRP Bundle | sc-522734 | 200 µg Ab, 40 µg BP | $354.00 | |||
BCKDK Antibody (F-10): m-IgG1 BP-HRP Bundle | sc-543664 | 200 µg Ab; 20 µg BP | $354.00 | |||
BCKDK Antibody (F-10) AC | sc-374424 AC | 500 µg/ml, 25% agarose | $416.00 | |||
BCKDK Antibody (F-10) HRP | sc-374424 HRP | 200 µg/ml | $316.00 | |||
BCKDK Antibody (F-10) FITC | sc-374424 FITC | 200 µg/ml | $330.00 | |||
BCKDK Antibody (F-10) PE | sc-374424 PE | 200 µg/ml | $343.00 | |||
BCKDK Antibody (F-10) Alexa Fluor® 488 | sc-374424 AF488 | 200 µg/ml | $357.00 | |||
BCKDK Antibody (F-10) Alexa Fluor® 546 | sc-374424 AF546 | 200 µg/ml | $357.00 | |||
BCKDK Antibody (F-10) Alexa Fluor® 594 | sc-374424 AF594 | 200 µg/ml | $357.00 | |||
BCKDK Antibody (F-10) Alexa Fluor® 647 | sc-374424 AF647 | 200 µg/ml | $357.00 | |||
BCKDK Antibody (F-10) Alexa Fluor® 680 | sc-374424 AF680 | 200 µg/ml | $357.00 | |||
BCKDK Antibody (F-10) Alexa Fluor® 790 | sc-374424 AF790 | 200 µg/ml | $357.00 |