BCAP Antibody (E-5): sc-515498
- BCAP Antibody (E-5) is a mouse monoclonal IgG1 κ BCAP antibody provided at 200 µg/ml
- raised against amino acids 552-797 mapping near the C-terminus of BCAP of human origin
- BCAP Antibody (E-5) is recommended for detection of BCAP isoforms 1-3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-BCAP Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BCAP Antibody (E-5) for WB applications. These reagents are now offered in bundles with BCAP Antibody (E-5) (see ordering information below).
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SEE ALSO...
BCAP Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects BCAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BCAP (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. The B cell adaptor for phosphoinositide 3-kinase (BCAP) plays a crucial role in B cell signaling by acting as a bridge between B cell receptor-associated kinases and the phosphoinositide 3-kinase (PIK3) pathway, which is essential for B cell activation and survival. Specifically, BCAP is phosphorylated by Syk, Btk, or Lyn kinases, creating binding sites for the p85 regulatory subunit of PIK3, thereby facilitating downstream signaling that influences cell proliferation and differentiation. BCAP mRNA expression is notably found in mouse spleen, thymus, liver, lung, macrophages, and B cell lines, highlighting its importance in immune responses. In humans, BCAP is located on chromosome 10q24.2, underscoring its evolutionary conservation and significance across species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BCAP Antibody (E-5) References:
- BCAP: the tyrosine kinase substrate that connects B cell receptor to phosphoinositide 3-kinase activation. | Okada, T., et al. 2000. Immunity. 13: 817-27. PMID: 11163197
- Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. | Inabe, K. and Kurosaki, T. 2002. Blood. 99: 584-9. PMID: 11781242
- Essential immunoregulatory role for BCAP in B cell development and function. | Yamazaki, T., et al. 2002. J Exp Med. 195: 535-45. PMID: 11877477
- Isolation of proteins that interact with the signal transduction molecule Dof and identification of a functional domain conserved between Dof and vertebrate BCAP. | Battersby, A., et al. 2003. J Mol Biol. 329: 479-93. PMID: 12767830
- Contribution of BCAP to maintenance of mature B cells through c-Rel. | Yamazaki, T. and Kurosaki, T. 2003. Nat Immunol. 4: 780-6. PMID: 12833156
- Enhanced NK-cell development and function in BCAP-deficient mice. | MacFarlane, AW., et al. 2008. Blood. 112: 131-40. PMID: 18337558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCAP Antibody (E-5) | sc-515498 | 200 µg/ml | $322.00 | |||
BCAP Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-531395 | 200 µg Ab; 10 µg BP | $361.00 | |||
BCAP Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-525073 | 200 µg Ab, 40 µg BP | $361.00 | |||
BCAP Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-544526 | 200 µg Ab; 20 µg BP | $361.00 | |||
BCAP Antibody (E-5) AC | sc-515498 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BCAP Antibody (E-5) HRP | sc-515498 HRP | 200 µg/ml | $322.00 | |||
BCAP Antibody (E-5) FITC | sc-515498 FITC | 200 µg/ml | $336.00 | |||
BCAP Antibody (E-5) PE | sc-515498 PE | 200 µg/ml | $349.00 | |||
BCAP Antibody (E-5) Alexa Fluor® 488 | sc-515498 AF488 | 200 µg/ml | $364.00 | |||
BCAP Antibody (E-5) Alexa Fluor® 546 | sc-515498 AF546 | 200 µg/ml | $364.00 | |||
BCAP Antibody (E-5) Alexa Fluor® 594 | sc-515498 AF594 | 200 µg/ml | $364.00 | |||
BCAP Antibody (E-5) Alexa Fluor® 647 | sc-515498 AF647 | 200 µg/ml | $364.00 | |||
BCAP Antibody (E-5) Alexa Fluor® 680 | sc-515498 AF680 | 200 µg/ml | $364.00 | |||
BCAP Antibody (E-5) Alexa Fluor® 790 | sc-515498 AF790 | 200 µg/ml | $364.00 |