BBOX1 Antibody (E-11): sc-373774
- BBOX1 Antibody (E-11) is a mouse monoclonal IgG1 κ BBOX1 antibody provided at 200 µg/ml
- raised against amino acids 47-346 mapping within an internal region of BBOX1 of human origin
- BBOX1 Antibody (E-11) is recommended for detection of BBOX1 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-BBOX1 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BBOX1 Antibody (E-11) for WB and IHC(P) applications. These reagents are now offered in bundles with BBOX1 Antibody (E-11) (see ordering information below).
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SEE ALSO...
BBOX1 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects BBOX1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BBOX1 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BBOX1, also known as butyrobetaine (gamma), 2-oxoglutarate dioxygenase 1, BBH, BBOX, or γ-BBH, is a crucial enzyme in the biosynthesis of L-carnitine, which is essential for the transport of activated fatty acids into the mitochondria for energy production. BBOX1 is primarily localized in the cytoplasm and is predominantly expressed in the kidney, where BBOX1 catalyzes the hydroxylation of γ-butyrobetaine to L-carnitine, a reaction that requires iron and ascorbate as cofactors. BBOX1′s importance extends beyond enzymatic function; BBOX1 also plays a role in the oxidative decarboxylation of α-ketoglutarate to succinate, linking the metabolism of amino acids and fatty acids. Proper functioning of BBOX1 is vital for maintaining cellular energy homeostasis, making BBOX1 a significant target for research in metabolic disorders and related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BBOX1 Antibody (E-11) References:
- Molecular cloning and characterization of the cDNA encoding the rat liver gamma-butyrobetaine hydroxylase. | Galland, S., et al. 1999. Biochim Biophys Acta. 1441: 85-92. PMID: 10526231
- Thyroid hormone controls carnitine status through modifications of gamma-butyrobetaine hydroxylase activity and gene expression. | Galland, S., et al. 2002. Cell Mol Life Sci. 59: 540-5. PMID: 11964131
- Microarray analysis of the effect of diesel exhaust particles on in vitro cultured macrophages. | Verheyen, GR., et al. 2004. Toxicol In Vitro. 18: 377-91. PMID: 15046786
- Carnitine deficiency and supplementation do not affect the gene expression of carnitine biosynthetic enzymes in rats. | Davis, AT. and Monroe, TJ. 2005. J Nutr. 135: 761-4. PMID: 15795431
- Genomic structure, alternative maturation and tissue expression of the human BBOX1 gene. | Rigault, C., et al. 2006. Biochim Biophys Acta. 1761: 1469-81. PMID: 17110165
- Multiple forms of gamma-butyrobetaine hydroxylase (EC 1.14.11.1). | Lindstedt, S. and Nordin, I. 1984. Biochem J. 223: 119-27. PMID: 6497835
- Gamma-butyrobetaine hydroxylase in human kidney. | Lindstedt, G., et al. 1982. Scand J Clin Lab Invest. 42: 477-85. PMID: 7156861
- Purification and characterization of the rat liver gamma-butyrobetaine hydroxylase. | Galland, S., et al. 1998. Mol Cell Biochem. 178: 163-8. PMID: 9546596
- Carnitine biosynthesis: identification of the cDNA encoding human gamma-butyrobetaine hydroxylase. | Vaz, FM., et al. 1998. Biochem Biophys Res Commun. 250: 506-10. PMID: 9753662
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BBOX1 Antibody (E-11) | sc-373774 | 200 µg/ml | $316.00 | |||
BBOX1 Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-529560 | 200 µg Ab; 10 µg BP | $354.00 | |||
BBOX1 Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-522495 | 200 µg Ab, 40 µg BP | $354.00 | |||
BBOX1 Antibody (E-11): m-IgG1 BP-HRP Bundle | sc-543579 | 200 µg Ab; 20 µg BP | $354.00 | |||
BBOX1 Antibody (E-11) AC | sc-373774 AC | 500 µg/ml, 25% agarose | $416.00 | |||
BBOX1 Antibody (E-11) HRP | sc-373774 HRP | 200 µg/ml | $316.00 | |||
BBOX1 Antibody (E-11) FITC | sc-373774 FITC | 200 µg/ml | $330.00 | |||
BBOX1 Antibody (E-11) PE | sc-373774 PE | 200 µg/ml | $343.00 | |||
BBOX1 Antibody (E-11) Alexa Fluor® 488 | sc-373774 AF488 | 200 µg/ml | $357.00 | |||
BBOX1 Antibody (E-11) Alexa Fluor® 546 | sc-373774 AF546 | 200 µg/ml | $357.00 | |||
BBOX1 Antibody (E-11) Alexa Fluor® 594 | sc-373774 AF594 | 200 µg/ml | $357.00 | |||
BBOX1 Antibody (E-11) Alexa Fluor® 647 | sc-373774 AF647 | 200 µg/ml | $357.00 | |||
BBOX1 Antibody (E-11) Alexa Fluor® 680 | sc-373774 AF680 | 200 µg/ml | $357.00 | |||
BBOX1 Antibody (E-11) Alexa Fluor® 790 | sc-373774 AF790 | 200 µg/ml | $357.00 |