Barttin Antibody (A-3): sc-365161

Barttin Antibody (A-3) is a mouse monoclonal IgG2b antibody that detects Barttin in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Barttin, encoded by the BSND gene, plays a crucial role as a b subunit for the chloride channels CLCKNA and CLCKNB, which are vital for maintaining electrolyte balance in the kidneys and inner ear. Barttin (A-3) antibody targets proteins primarily located in the basolateral membranes of renal tubules and potassium-secreting epithelia of the inner ear, where Barttin facilitates chloride ion transport essential for renal salt reabsorption and potassium recycling. Mutations in the BSND gene can lead to Bartter syndrome, a genetic disorder characterized by electrolyte imbalances and sensorineural deafness, highlighting Barttin′s importance in both renal function and auditory health. The interaction of Barttin with chloride channels is critical, as Barttin enhances the currents through these heteromeric channels, and any disruption in this interaction can have significant physiological consequences. Anti-Barttin antibody (A-3) serves as an invaluable tool for researchers studying the molecular mechanisms underlying Bartter syndrome and related disorders, providing insights into Barttin′s function and role in cellular homeostasis.

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Barttin Antibody (A-3) References:

1. Barttin increases surface expression and changes current properties of ClC-K channels.  |  Waldegger, S., et al. 2002. Pflugers Arch. 444: 411-8. PMID: 12111250
2. Regulation of CLC-Ka/barttin by the ubiquitin ligase Nedd4-2 and the serum- and glucocorticoid-dependent kinases.  |  Embark, HM., et al. 2004. Kidney Int. 66: 1918-25. PMID: 15496163
3. Barttin modulates trafficking and function of ClC-K channels.  |  Scholl, U., et al. 2006. Proc Natl Acad Sci U S A. 103: 11411-6. PMID: 16849430
4. Barttin binds to the outer lateral surface of the ClC-K2 chloride channel.  |  Tajima, M., et al. 2007. Biochem Biophys Res Commun. 362: 858-64. PMID: 17767918
5. Mechanisms of Disease: the kidney-specific chloride channels ClCKA and ClCKB, the Barttin subunit, and their clinical relevance.  |  Krämer, BK., et al. 2008. Nat Clin Pract Nephrol. 4: 38-46. PMID: 18094726
6. Disease-causing dysfunctions of barttin in Bartter syndrome type IV.  |  Janssen, AG., et al. 2009. J Am Soc Nephrol. 20: 145-53. PMID: 18776122
7. Modulation of ClC-K channel function by the accessory subunit barttin.  |  Lang, F. 2010. J Am Soc Nephrol. 21: 1238-9. PMID: 20595683
8. Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.  |  Steinke, KV., et al. 2015. J Biol Chem. 290: 17390-400. PMID: 26013830
9. Carboxyl-terminal Truncations of ClC-Kb Abolish Channel Activation by Barttin Via Modified Common Gating and Trafficking.  |  Stölting, G., et al. 2015. J Biol Chem. 290: 30406-16. PMID: 26453302
10. Mouse genetics reveals Barttin as a genetic modifier of Joubert syndrome.  |  Ramsbottom, SA., et al. 2020. Proc Natl Acad Sci U S A. 117: 1113-1118. PMID: 31879347

The preferred Barttin antibody is Barttin Antibody (A-1): sc-271867.