BART1 Antibody (6): sc-136389
- BART1 Antibody (6) is a mouse monoclonal IgG1 κ BART1 antibody provided at 200 µg/ml
- raised against amino acids 22-134 of BART1 of human origin
- recommended for detection of BART1 of mouse, rat and human origin by WB, IP and IF
- available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BART1 Antibody (6) for WB applications. These reagents are now offered in bundles with BART1 Antibody (6) (see ordering information below).
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BART1 Antibody (6) is a mouse monoclonal IgG1 kappa light chain antibody that detects BART1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). BART1 (6) antibody is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP) conjugates. BART1 protein, also known as Binder of ARL2 protein 1 or ARL2BP, is a 163 amino acid protein that plays a crucial role in cellular processes by interacting with GTP-bound ADP-ribosylation factor-like protein 2 (ARL2) complexes. This interaction modulates ARL2 activity, which is essential for regulating vesicular trafficking pathways and activating phospholipase D. BART1 is predominantly located in the cytosol but can translocate to the mitochondria, where BART1 binds to the adenine nucleotide transporter in the presence of ARL2, highlighting BART1′s role in cellular energy metabolism and signaling. Additionally, BART1 is produced in two isoforms due to alternative splicing, which may contribute to BART1′s functional diversity in various cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.
Download thiomersal SDS
BART1 Antibody (6) References:
- The ARF-like 2 (ARL2)-binding protein, BART. Purification, cloning, and initial characterization. | Sharer, JD. and Kahn, RA. 1999. J Biol Chem. 274: 27553-61. PMID: 10488091
- ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins. | Van Valkenburgh, H., et al. 2001. J Biol Chem. 276: 22826-37. PMID: 11303027
- ARL2 and BART enter mitochondria and bind the adenine nucleotide transporter. | Sharer, JD., et al. 2002. Mol Biol Cell. 13: 71-83. PMID: 11809823
- Arl2 and Arl3 regulate different microtubule-dependent processes. | Zhou, C., et al. 2006. Mol Biol Cell. 17: 2476-87. PMID: 16525022
- Crystal structure of the ARL2-GTP-BART complex reveals a novel recognition and binding mode of small GTPase with effector. | Zhang, T., et al. 2009. Structure. 17: 602-10. PMID: 19368893
- 1H, 13C and 15N resonance assignments for Binder of Arl2, BART. | Bailey, LK., et al. 2009. Biomol NMR Assign. 3: 33-6. PMID: 19636941
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BART1 Antibody (6) | sc-136389 | 200 µg/ml | $322.00 | |||
BART1 Antibody (6): m-IgG Fc BP-HRP Bundle | sc-528819 | 200 µg Ab; 10 µg BP | $361.00 | |||
BART1 Antibody (6): m-IgGκ BP-HRP Bundle | sc-521363 | 200 µg Ab, 40 µg BP | $361.00 | |||
BART1 Antibody (6): m-IgG1 BP-HRP Bundle | sc-543150 | 200 µg Ab; 20 µg BP | $361.00 | |||
BART1 Antibody (6) AC | sc-136389 AC | 500 µg/ml, 25% agarose | $424.00 | |||
BART1 Antibody (6) HRP | sc-136389 HRP | 200 µg/ml | $322.00 |