BAP31 Antibody (B-10): sc-365347

BAP31 Antibody (B-10) is a mouse monoclonal IgG1 antibody that detects BAP31 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BAP31 is a crucial integral membrane protein located in the endoplasmic reticulum, where BAP31 plays a significant role in apoptosis regulation. BAP31 forms a protein complex that connects apoptosis-initiating caspases, like procaspase-8, with anti-apoptotic regulators such as Bcl-2 and Bcl-XL, thereby influencing cell survival and death pathways. The cytosolic domain of BAP31 contains two identical caspase recognition sites, which are specifically cleaved by initiator caspases, leading to the generation of a p20 fragment during apoptosis. This fragment remains embedded in the membrane and acts as a potent inducer of cell death when ectopically expressed. The cleavage of BAP31 is vital for the execution of cytoplasmic apoptotic events, including membrane fragmentation, and facilitates the interaction between mitochondria and the endoplasmic reticulum during Fas-mediated apoptosis. The BAP31 gene is ubiquitously expressed across murine tissues and is located on the X chromosome in both mouse and human, highlighting BAP31′s evolutionary conservation and importance in cellular processes. Anti-BAP31 antibody (B-10) is an essential tool for researchers studying apoptosis and related signaling pathways.

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BAP31 Antibody (B-10) References:

1. Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils.  |  Zen, K., et al. 2004. J Biol Chem. 279: 44924-30. PMID: 15294914
2. Monoclonal antibodies against human BAP31 for immunocytochemistry.  |  Song, C., et al. 2009. Hybridoma (Larchmt). 28: 177-81. PMID: 19519245
3. Epitope Mapping of Antibodies Suggests the Novel Membrane Topology of B-Cell Receptor Associated Protein 31 on the Cell Surface of Embryonic Stem Cells: The Novel Membrane Topology of BAP31.  |  Kim, WT., et al. 2015. PLoS One. 10: e0130670. PMID: 26102500
4. BAP31, a newly defined cancer/testis antigen, regulates proliferation, migration, and invasion to promote cervical cancer progression.  |  Dang, E., et al. 2018. Cell Death Dis. 9: 791. PMID: 30022068
5. Induction of Liver Steatosis in BAP31-Deficient Mice Burdened with Tunicamycin-Induced Endoplasmic Reticulum Stress.  |  Wu, Z., et al. 2018. Int J Mol Sci. 19: PMID: 30081561
6. Inhibition of BAP31 expression inhibits cervical cancer progression by suppressing metastasis and inducing intrinsic and extrinsic apoptosis.  |  Wang, A., et al. 2019. Biochem Biophys Res Commun. 508: 499-506. PMID: 30503502
7. BAP31 deficiency contributes to the formation of amyloid-β plaques in Alzheimer's disease by reducing the stability of RTN3.  |  Wang, T., et al. 2019. FASEB J. 33: 4936-4946. PMID: 30596517
8. BAP31 Inhibits Cell Adaptation to ER Stress Conditions, Negatively Regulating Autophagy Induction by Interaction with STX17.  |  Machihara, K. and Namba, T. 2019. Cells. 8: PMID: 31671609
9. An Intrabody against B-Cell Receptor-Associated Protein 31 (BAP31) Suppresses the Glycosylation of the Epithelial Cell-Adhesion Molecule (EpCAM) via Affecting the Formation of the Sec61-Translocon-Associated Protein (TRAP) Complex.  |  Wang, T., et al. 2023. Int J Mol Sci. 24: PMID: 37834237
10. Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31.  |  Annaert, WG., et al. 1997. J Cell Biol. 139: 1397-410. PMID: 9396746

The preferred BAP31 antibody is BAP31 Antibody (D-6): sc-393810.