Baculovirus gp64 Antibody (AcV1): sc-65498

Baculovirus gp64 Antibody (AcV1) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect baculovirus envelope glycoprotein gp64, also known as homotrimeric baculoviridae membrane glycoprotein gp64 or Major envelope glycoprotein GP64, from Autographa californica multiple nucleopolyhedrovirus (AcNPV) origin using western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM) applications. GP64 is a crucial protein involved in the viral life cycle, primarily responsible for mediating the fusion of the viral envelope with host cell membranes, which is essential for viral entry and infection. Located on the surface of both infected cells and budded virions, GP64 facilitates the attachment and fusion processes that allow the virus to disseminate within the host. Structurally, GP64 is a trimeric phosphoglycoprotein featuring multiple N-linked glycosylation sites and hydrophobic termini that anchor GP64 firmly within the viral membrane, ensuring stability and functionality. Post-translational modifications, particularly glycosylation, are vital for proper folding and immune recognition of GP64, enhancing viral infectivity and interaction with host cell receptors. GP64 interacts with various host proteins to orchestrate the entry process, making GP64 a key target for studying baculovirus-host interactions and developing antiviral strategies. Anti-gp64 antibody (AcV1) is available in both non-conjugated form and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile tools for researchers investigating the mechanisms of baculovirus infection, gene delivery systems, and protein expression studies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Baculovirus gp64 Antibody (AcV1) References:

1. A discrete stage of baculovirus GP64-mediated membrane fusion.  |  Kingsley, DH., et al. 1999. Mol Biol Cell. 10: 4191-200. PMID: 10588652
2. Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion.  |  Blissard, GW. and Wenz, JR. 1992. J Virol. 66: 6829-35. PMID: 1404622
3. The baculovirus GP64 protein mediates highly stable infectivity of a human respiratory syncytial virus lacking its homologous transmembrane glycoproteins.  |  Oomens, AG. and Wertz, GW. 2004. J Virol. 78: 124-35. PMID: 14671094
4. Baculovirus gp64 gene expression: analysis of sequences modulating early transcription and transactivation by IE1.  |  Blissard, GW. and Rohrmann, GF. 1991. J Virol. 65: 5820-7. PMID: 1920618
5. The baculovirus GP64 envelope fusion protein: synthesis, oligomerization, and processing.  |  Oomens, AG., et al. 1995. Virology. 209: 592-603. PMID: 7778291
6. Overlapping TATA-dependent and TATA-independent early promoter activities in the baculovirus gp64 envelope fusion protein gene.  |  Kogan, PH., et al. 1995. J Virol. 69: 1452-61. PMID: 7853477
7. A baculovirus gp64 early promoter is activated by host transcription factor binding to CACGTG and GATA elements.  |  Kogan, PH. and Blissard, GW. 1994. J Virol. 68: 813-22. PMID: 8289385
8. Late promoter selection in the baculovirus gp64 envelope fusion protein gene.  |  Garrity, DB., et al. 1997. Virology. 231: 167-81. PMID: 9168879
9. Baculovirus gp64 gene expression: negative regulation by a minicistron.  |  Chang, MJ. and Blissard, GW. 1997. J Virol. 71: 7448-60. PMID: 9311824

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Baculovirus gp64 Antibody (AcV1) has 16 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Baculovirus gp64 Antibody (AcV1) Specifications

Alternate Product Name: Major envelope glycoprotein 64 kDa (gp64)

Product Clone Name: AcV1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Baculovirus gp64

Epitope Species: AcNPV extracellular nonoccluded virus (NOV)

Product Reactivity/Detection: baculovirus envelope protein gp64

Species Reactivity/Detection: AcNPV

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Baculovirus gp64 Antibody (AcV1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Baculovirus gp64 antibody is Baculovirus gp64 Antibody (AcV5): sc-65499.