Date published: 2025-10-7

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B23/Nucleophosmin Antibody (NA24): sc-53175

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Datasheets
  • B23/Nucleophosmin Antibody (NA24) is a mouse monoclonal IgG1 κ, cited in 17 publications, provided at 200 µg/ml
  • raised against a recombinant protein corresponding to the N-terminus of B23 of human origin
  • recommended for detection of B23 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See B23/Nucleophosmin (E-3): sc-271737 for B23/Nucleophosmin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for B23/Nucleophosmin Antibody (NA24) for WB and IHC(P) applications. These reagents are now offered in bundles with B23/Nucleophosmin Antibody (NA24) (see ordering information below).

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B23 Antibody (NA24) is a mouse monoclonal IgG1 antibody that detects B23 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. B23, also known as nucleophosmin, plays a crucial role in ribosomal assembly and the transport of ribosomal RNA, making B23 essential for protein synthesis and cellular function. B23 is predominantly located in the nucleolus, where B23 is involved in the maturation of ribosomal subunits, and proper localization is vital for maintaining efficient ribosome biogenesis. Additionally, B23 undergoes significant post-translational modifications, particularly phosphorylation by Cdc2 kinase during mitosis, which can influence B23′s activity and interactions with other cellular components. Anti-B23 antibody (NA24) is an invaluable tool for researchers studying nucleolar dynamics, ribosome assembly, and the regulatory mechanisms of cell cycle progression, providing insights into the fundamental processes that underpin cellular growth and proliferation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

B23/Nucleophosmin Antibody (NA24)

sc-53175
200 µg/ml
$316.00

B23/Nucleophosmin Antibody (NA24): m-IgG Fc BP-HRP Bundle

sc-536903
200 µg Ab; 10 µg BP
$354.00

B23/Nucleophosmin Antibody (NA24): m-IgGκ BP-HRP Bundle

sc-534075
200 µg Ab; 40 µg BP
$354.00

B23/Nucleophosmin Antibody (NA24): m-IgG1 BP-HRP Bundle

sc-544887
200 µg Ab; 20 µg BP
$354.00

I am using B23 (NA24): sc-53175 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from An antibody that will make you happy!The nucleolin (B23) is always my favorite protein because of its distribution in the cell, it gives punctuate spots in the nucleus which looks great in immuno-fluorescence. The antibody gave clean bands at around 40kda in human cell lines as well as mouse cell line(see the blot). Which is expected size of the proteins, the additional bands may be isoforms or post transnational modification of the protein. Highly recommend this antibody.
Date published: 2017-11-14
Rated 5 out of 5 by from For WBFor WB, dilution 1:1000 gives a very good signal.
Date published: 2016-06-28
Rated 5 out of 5 by from Published IHC data for use in human bonePublished IHC data for use in human bone. -SCBT Publication Review
Date published: 2015-06-04
Rated 5 out of 5 by from Published data for IF in HeLa cellsPublished data for IF in HeLa cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Exceptional immunoperoxidase nucleolar andExceptional immunoperoxidase nucleolar and nuclear staining in formalin fixed, paraffin-embedded human vulva/anal skin tissue. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Worked well for WB in human B23 transfectedWorked well for WB in human B23 transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Clean band observed in WB in HeLa whole cellClean band observed in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Band of interest observed in WB in nonBand of interest observed in WB in non-transfected 293T, human B23 transfected 293T and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-01-31
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B23/Nucleophosmin Antibody (NA24) is rated 5.0 out of 5 by 8.
  • y_2025, m_9, d_24, h_6
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