ATP6E Antibody (G-3): sc-514143
- ATP6E Antibody (G-3) is a mouse monoclonal IgG2a κ ATP6E antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 158-184 within an internal region of ATP6E of human origin
- ATP6E Antibody (G-3) is recommended for detection of ATP6E of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ATP6E Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ATP6E Antibody (G-3) for WB applications. These reagents are now offered in bundles with ATP6E Antibody (G-3) (see ordering information below).
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ATP6E Antibody (G-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects ATP6E of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ATP6E (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATP6E, also known as V-ATPase E, is a crucial component of the vacuolar-type H+-ATPase (V-ATPase) complex, which plays a vital role in the acidification of eukaryotic intracellular organelles. ATP6E is primarily located in the membranes of lysosomes, endosomes, and other organelles, where ATP6E is essential for maintaining the acidic environment necessary for various cellular processes, including protein degradation, nutrient storage, and ion homeostasis. Proper functioning of ATP6E is critical for cellular health, as disruptions in ATP6E activity can lead to impaired organelle function and contribute to various diseases, including cancer and neurodegenerative disorders. ATP6E (G-3) monoclonal antibody provides a reliable tool for researchers studying the dynamics of intracellular pH regulation and the role of V-ATPase in cellular physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ATP6E Antibody (G-3) References:
- The vacuolar (H+)-ATPases--nature's most versatile proton pumps. | Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
- The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22. | Baud, V., et al. 1994. Hum Mol Genet. 3: 335-9. PMID: 8004105
- Three vha genes encode proteolipids of Caenorhabditis elegans vacuolar-type ATPase. Gene structures and preferential expression in an H-shaped excretory cell and rectal cells. | Oka, T., et al. 1997. J Biol Chem. 272: 24387-92. PMID: 9305897
- Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules. | Ludwig, J., et al. 1998. J Biol Chem. 273: 10939-47. PMID: 9556572
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP6E Antibody (G-3) | sc-514143 | 200 µg/ml | $316.00 | |||
ATP6E Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-531042 | 200 µg Ab; 10 µg BP | $354.00 | |||
ATP6E Antibody (G-3): m-IgGκ BP-HRP Bundle | sc-524621 | 200 µg Ab, 40 µg BP | $354.00 | |||
ATP6E Antibody (G-3): m-IgG2a BP-HRP Bundle | sc-547670 | 200 µg Ab; 10 µg BP | $354.00 | |||
ATP6E Antibody (G-3) AC | sc-514143 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ATP6E Antibody (G-3) HRP | sc-514143 HRP | 200 µg/ml | $316.00 | |||
ATP6E Antibody (G-3) FITC | sc-514143 FITC | 200 µg/ml | $330.00 | |||
ATP6E Antibody (G-3) PE | sc-514143 PE | 200 µg/ml | $343.00 | |||
ATP6E Antibody (G-3) Alexa Fluor® 488 | sc-514143 AF488 | 200 µg/ml | $357.00 | |||
ATP6E Antibody (G-3) Alexa Fluor® 546 | sc-514143 AF546 | 200 µg/ml | $357.00 | |||
ATP6E Antibody (G-3) Alexa Fluor® 594 | sc-514143 AF594 | 200 µg/ml | $357.00 | |||
ATP6E Antibody (G-3) Alexa Fluor® 647 | sc-514143 AF647 | 200 µg/ml | $357.00 | |||
ATP6E Antibody (G-3) Alexa Fluor® 680 | sc-514143 AF680 | 200 µg/ml | $357.00 | |||
ATP6E Antibody (G-3) Alexa Fluor® 790 | sc-514143 AF790 | 200 µg/ml | $357.00 | |||
ATP6E (G-3) Neutralizing Peptide | sc-514143 P | 100 µg/0.5 ml | $68.00 |