ATP6AP1 Antibody (E-10): sc-515607
- ATP6AP1 Antibody (E-10) is a mouse monoclonal IgG1 κ ATP6AP1 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 51-68 near the N-terminus of ATP6AP1 of human origin
- ATP6AP1 Antibody (E-10) is recommended for detection of ATP6AP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ATP6AP1 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ATP6AP1 Antibody (E-10) for WB applications. These reagents are now offered in bundles with ATP6AP1 Antibody (E-10) (see ordering information below).
QUICK LINKS
ATP6AP1 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATP6AP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ATP6AP1 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATP6AP1, also known as ATPase, H+ transporting, lysosomal accessory protein 1, plays a crucial role in the function of the vacuolar-type H+-ATPase (V-ATPase), a multisubunit enzyme essential for the acidification of eukaryotic intracellular organelles. ATP6AP1 is primarily located in the cytoplasmic region of endocrine and neuronal cells, where ATP6AP1 serves as an accessory protein that targets the V-ATPase enzyme to specialized vacuolar systems. ATP6AP1′s interaction with the V0 domain subunits is vital for the proper assembly and function of the V-ATPase complex, and any disruption in this interaction can lead to impaired bone resorption in osteoclasts, highlighting ATP6AP1′s significant role in maintaining bone health. Anti-ATP6AP1 antibody (E-10) enables researchers to effectively study the dynamics of V-ATPase assembly and its implications in various physiological and pathological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ATP6AP1 Antibody (E-10) References:
- ATP6AP1 deficiency causes an immunodeficiency with hepatopathy, cognitive impairment and abnormal protein glycosylation. | Jansen, EJ., et al. 2016. Nat Commun. 7: 11600. PMID: 27231034
- Prenatal and postnatal phenotype of a pathologic variant in the ATP6AP1 gene. | Tvina, A., et al. 2020. Eur J Med Genet. 63: 103881. PMID: 32058063
- Structure of V-ATPase from the mammalian brain. | Abbas, YM., et al. 2020. Science. 367: 1240-1246. PMID: 32165585
- Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly. | Wang, L., et al. 2020. Mol Cell. 80: 501-511.e3. PMID: 33065002
- Prognostic and immunological value of ATP6AP1 in breast cancer: implications for SARS-CoV-2. | Wang, J., et al. 2021. Aging (Albany NY). 13: 16904-16921. PMID: 34228637
- SARS-CoV-2 non-structural protein 6 triggers NLRP3-dependent pyroptosis by targeting ATP6AP1. | Sun, X., et al. 2022. Cell Death Differ. 29: 1240-1254. PMID: 34997207
- ATP6AP1 is a potential prognostic biomarker and is associated with iron metabolism in breast cancer. | Tian, Y., et al. 2022. Front Genet. 13: 958290. PMID: 36147483
- Evaluation of PEN2-ATP6AP1 axis as an antiparasitic target for metformin based on phylogeny analysis and molecular docking. | Liu, C., et al. 2023. Mol Biochem Parasitol. 255: 111580. PMID: 37473813
- Utility of sequencing for ATP6AP1 and ATP6AP2 to distinguish between atypical granular cell tumor with junctional component and melanoma. | Warren, SJ. and Alomari, AK. 2023. J Cutan Pathol. 50: 1014-1019. PMID: 37565535
- Lysosomal gene ATP6AP1 promotes doxorubicin resistance via up-regulating autophagic flux in breast cancer. | Fei, Y., et al. 2024. Cancer Cell Int. 24: 394. PMID: 39627767
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP6AP1 Antibody (E-10) | sc-515607 | 200 µg/ml | $316.00 | |||
ATP6AP1 Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-531437 | 200 µg Ab; 10 µg BP | $354.00 | |||
ATP6AP1 Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-525126 | 200 µg Ab, 40 µg BP | $354.00 | |||
ATP6AP1 Antibody (E-10): m-IgG1 BP-HRP Bundle | sc-544555 | 200 µg Ab; 20 µg BP | $354.00 | |||
ATP6AP1 Antibody (E-10) AC | sc-515607 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ATP6AP1 Antibody (E-10) HRP | sc-515607 HRP | 200 µg/ml | $316.00 | |||
ATP6AP1 Antibody (E-10) FITC | sc-515607 FITC | 200 µg/ml | $330.00 | |||
ATP6AP1 Antibody (E-10) PE | sc-515607 PE | 200 µg/ml | $343.00 | |||
ATP6AP1 Antibody (E-10) Alexa Fluor® 488 | sc-515607 AF488 | 200 µg/ml | $357.00 | |||
ATP6AP1 Antibody (E-10) Alexa Fluor® 546 | sc-515607 AF546 | 200 µg/ml | $357.00 | |||
ATP6AP1 Antibody (E-10) Alexa Fluor® 594 | sc-515607 AF594 | 200 µg/ml | $357.00 | |||
ATP6AP1 Antibody (E-10) Alexa Fluor® 647 | sc-515607 AF647 | 200 µg/ml | $357.00 | |||
ATP6AP1 Antibody (E-10) Alexa Fluor® 680 | sc-515607 AF680 | 200 µg/ml | $357.00 | |||
ATP6AP1 Antibody (E-10) Alexa Fluor® 790 | sc-515607 AF790 | 200 µg/ml | $357.00 | |||
ATP6AP1 (E-10) Neutralizing Peptide | sc-515607 P | 100 µg/0.5 ml | $68.00 |