ATP6AP1 Antibody (85.1): sc-81886

ATP6AP1 Antibody (85.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATP6AP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-ATP6AP1 antibody (85.1) is available in a non-conjugated form, specifically designed for researchers seeking to study the vacuolar-type H+-ATPase (V-ATPase) complex. V-ATPase plays a crucial role in the acidification of eukaryotic intracellular organelles, which is essential for various cellular processes, including protein degradation, nutrient storage, and pH regulation. The V-ATPase is composed of two main domains: the peripheral V1 domain, responsible for ATP hydrolysis, and the integral V0 domain, which facilitates proton translocation across membranes. The V1 domain consists of nine subunits (A-H), while the V0 domain is made up of five subunits (a, d, c, c′, and c"). ATP6AP1, also known as 16A, CF2, Ac45, XAP3, ATP6S1, and VATPS1, is a type I transmembrane accessory protein predominantly expressed in endocrine and neuronal cells. ATP6AP1 plays a vital role in targeting the V-ATPase enzyme to specialized vacuolar systems, and through its cytoplasmic tail, ATP6AP1 interacts with the V0 domain subunits. Disruption of this interaction in osteoclasts has been shown to impair bone resorption, highlighting ATP6AP1′s importance in maintaining proper osteoclastic function and bone health.

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ATP6AP1 Antibody (85.1) References:

1. Biosynthesis of the vacuolar H+-ATPase accessory subunit Ac45 in Xenopus pituitary.  |  Holthuis, JC., et al. 1999. Eur J Biochem. 262: 484-91. PMID: 10336633
2. The fate of newly synthesized V-ATPase accessory subunit Ac45 in the secretory pathway.  |  Schoonderwoert, VT., et al. 2002. Eur J Biochem. 269: 1844-53. PMID: 11952786
3. Targeted disruption of the mouse gene encoding the V-ATPase accessory subunit Ac45.  |  Schoonderwoert, VT. and Martens, GJ. 2002. Mol Membr Biol. 19: 67-71. PMID: 11989824
4. Structural gene organization and evolutionary aspects of the V-ATPase accessory subunit Ac45.  |  Schoonderwoert, VT. and Martens, GJ. 2002. Biochim Biophys Acta. 1574: 245-54. PMID: 11997089
5. Structure and function of V-ATPases in osteoclasts: potential therapeutic targets for the treatment of osteolysis.  |  Xu, J., et al. 2007. Histol Histopathol. 22: 443-54. PMID: 17290355
6. Cytoplasmic terminus of vacuolar type proton pump accessory subunit Ac45 is required for proper interaction with V(0) domain subunits and efficient osteoclastic bone resorption.  |  Feng, H., et al. 2008. J Biol Chem. 283: 13194-204. PMID: 18227071
7. ATP6AP1-CDG: Follow-up and female phenotype.  |  Lipiński, P., et al. 2020. JIMD Rep. 53: 80-82. PMID: 32395412
8. ATP6AP1 as a potential prognostic biomarker in CRC by comprehensive analysis and verification.  |  Zhang, S., et al. 2024. Sci Rep. 14: 4018. PMID: 38369634
9. The rapid proximity labeling system PhastID identifies ATP6AP1 as an unconventional GEF for Rheb.  |  Feng, R., et al. 2024. Cell Res. 34: 355-369. PMID: 38448650
10. A novel accessory subunit for vacuolar H(+)-ATPase from chromaffin granules.  |  Supek, F., et al. 1994. J Biol Chem. 269: 24102-6. PMID: 7929063
11. Chromaffin granule membrane glycoprotein IV is identical with Ac45, a membrane-integral subunit of the granule's H(+)-ATPase.  |  Getlawi, F., et al. 1996. Neurosci Lett. 219: 13-6. PMID: 8961292
12. Intracellular trafficking of the vacuolar H+-ATPase accessory subunit Ac45.  |  Jansen, EJ., et al. 1998. J Cell Sci. 111 (Pt 20): 2999-3006. PMID: 9739073

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATP6AP1 Antibody (85.1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-ATP6AP1 Antibody (85.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATP6AP1 Antibody (85.1) Specifications

Alternate Product Name: ATP6AP1

Product Clone Name: 85.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 51-151

Epitope Target: ATP6AP1

Epitope Species: human

Product Reactivity/Detection: ATP6AP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for ATP6AP1 Antibody (85.1): N/A

The preferred ATP6AP1 antibody is ATP6AP1 Antibody (E-10): sc-515607.