ATP5B Antibody (F-1): sc-166462

ATP5B Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects ATP5B in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ATP5B, also known as ATPMB or ATPSB, is a crucial 529 amino acid protein that functions as a subunit of the F0 complex of mitochondrial ATP synthase, essential for ATP production during oxidative phosphorylation. ATP5B is located in the mitochondrial membrane, forming part of the proton channel that facilitates ATP synthesis by utilizing the electrochemical gradient generated across the inner mitochondrial membrane. Proper functioning of ATP5B is critical for cellular energy metabolism, and dysregulation can lead to various metabolic disorders. ATP5B is encoded by a nuclear gene and assembles with other subunits encoded by both mitochondrial and nuclear genes, highlighting the intricate coordination required for mitochondrial function. ATP5B mRNA expression varies significantly among different tissues, with high levels found in the heart, moderate levels in skeletal muscle, and low levels in the liver and kidney, indicating specialized roles in energy-demanding tissues. ATP5B gene regulation by Ets family transcription factors suggests these factors may influence gene expression in rapidly proliferating cells, emphasizing ATP5B′s importance in cellular energy dynamics.

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ATP5B Antibody (F-1) References:

1. ATP5B and ETFB metabolic markers in children with congenital hydronephrosis.  |  Zhao, Q., et al. 2016. Mol Med Rep. 14: 5111-5115. PMID: 27840937
2. Profiling of rotavirus 3'UTR-binding proteins reveals the ATP synthase subunit ATP5B as a host factor that supports late-stage virus replication.  |  Ren, L., et al. 2019. J Biol Chem. 294: 5993-6006. PMID: 30770472
3. Targeted inhibition of ATP5B gene prevents bone erosion in collagen-induced arthritis by inhibiting osteoclastogenesis.  |  Xu, Y., et al. 2021. Pharmacol Res. 165: 105458. PMID: 33515708
4. ATP5B promotes the metastasis and growth of gastric cancer by activating the FAK/AKT/MMP2 pathway.  |  Wang, X., et al. 2021. FASEB J. 35: e20649. PMID: 33715234
5. Integrated Analyses Reveal the Multi-Omics and Prognostic Characteristics of ATP5B in Breast Cancer.  |  Liu, M., et al. 2021. Front Genet. 12: 652474. PMID: 34122507
6. Disrupting metformin adaptation of liver cancer cells by targeting the TOMM34/ATP5B axis.  |  Jin, P., et al. 2022. EMBO Mol Med. 14: e16082. PMID: 36321555
7. Mitochondrial iron overload mediated by cooperative transfer of plasma membrane ATP5B and TFR2 to mitochondria triggers hepatic insulin resistance under PFOS exposure.  |  Wang, J., et al. 2023. Ecotoxicol Environ Saf. 253: 114662. PMID: 36801541
8. Adamantaniline Derivatives Target ATP5B to Inhibit Translation of Hypoxia Inducible Factor-1α.  |  Li, H., et al. 2023. Adv Sci (Weinh). 10: e2301071. PMID: 37401167
9. ATP synthase subunit ATP5B interacts with TGEV Nsp2 and acts as a negative regulator of TGEV replication.  |  Wang, Y., et al. 2024. Virulence. 15: 2397492. PMID: 39239724
10. Mitochondrial MOF regulates energy metabolism in heart failure via ATP5B hyperacetylation.  |  Hu, Y., et al. 2024. Cell Rep. 43: 114839. PMID: 39392752

The preferred ATP5B antibody is ATP5B Antibody (E-1): sc-55597.