ATP5B Antibody (E-1): sc-55597

ATP5B Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATP5B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ATP5B (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATP5B, also known as ATP synthase F1 subunit beta, plays a crucial role in mitochondrial function by forming part of the ATP synthase complex, which is essential for ATP production during oxidative phosphorylation. ATP5B is located in the mitochondrial membrane, where ATP5B contributes to the F0 complex, a vital component that facilitates proton translocation across the membrane, driving ATP synthesis from adenosine diphosphate and inorganic phosphate. Proper functioning of ATP5B is critical for cellular energy metabolism, and dysregulation can lead to various metabolic disorders and mitochondrial diseases. ATP5B is encoded by a nuclear gene and assembles with other subunits encoded by both mitochondrial and nuclear genes, highlighting the intricate coordination required for mitochondrial biogenesis and function. ATP5B mRNA expression varies significantly among different tissues, with high levels found in the heart, moderate levels in skeletal muscle, and lower levels in the liver and kidney, indicating ATP5B importance in energy-demanding tissues.

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ATP5B Antibody (E-1) References:

1. Understanding ATP synthesis: structure and mechanism of the F1-ATPase (Review).  |  Leyva, JA., et al. 2003. Mol Membr Biol. 20: 27-33. PMID: 12745923
2. Kinetic studies of ATP synthase: the case for the positional change mechanism.  |  LaNoue, KF. and Duszynski, J. 1992. J Bioenerg Biomembr. 24: 499-506. PMID: 1429543
3. Regulation of the mitochondrial ATPase in situ in cardiac muscle: role of the inhibitor subunit.  |  Rouslin, W. 1991. J Bioenerg Biomembr. 23: 873-88. PMID: 1838111
4. ATP synthase subunit-β down-regulation aggravates diabetic nephropathy.  |  Guan, SS., et al. 2015. Sci Rep. 5: 14561. PMID: 26449648
5. The human ATP synthase beta subunit gene: sequence analysis, chromosome assignment, and differential expression.  |  Neckelmann, N., et al. 1989. Genomics. 5: 829-43. PMID: 2687158
6. ATP synthase β-subunit abnormality in pancreas islets of rats with polycystic ovary syndrome and type 2 diabetes mellitus.  |  Li, W., et al. 2017. J Huazhong Univ Sci Technolog Med Sci. 37: 210-216. PMID: 28397049
7. Human F1-ATPase: molecular cloning of cDNA for the beta subunit.  |  Ohta, S. and Kagawa, Y. 1986. J Biochem. 99: 135-41. PMID: 2870059
8. FUS interacts with ATP synthase beta subunit and induces mitochondrial unfolded protein response in cellular and animal models.  |  Deng, J., et al. 2018. Proc Natl Acad Sci U S A. 115: E9678-E9686. PMID: 30249657
9. ATP synthase subunit ATP5B interacts with TGEV Nsp2 and acts as a negative regulator of TGEV replication.  |  Wang, Y., et al. 2024. Virulence. 15: 2397492. PMID: 39239724
10. ETS transcription factors regulate the expression of the gene for the human mitochondrial ATP synthase beta-subunit.  |  Villena, JA., et al. 1994. J Biol Chem. 269: 32649-54. PMID: 7798271
11. Energy transduction in ATP synthase.  |  Elston, T., et al. 1998. Nature. 391: 510-3. PMID: 9461222
12. Energy transduction in the F1 motor of ATP synthase.  |  Wang, H. and Oster, G. 1998. Nature. 396: 279-82. PMID: 9834036

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATP5B Antibody (E-1) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-ATP5B Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATP5B Antibody (E-1) Specifications

Alternate Product Name: ATP5F1B

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 230-529

Epitope Target: ATP5B

Epitope Species: human

Product Reactivity/Detection: ATP5B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for ATP5B Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin