ATIII Antibody (H-7): sc-271987

ATIII Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATIII protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ATIII monoclonal antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Antithrombin III (ATIII) plays a vital role in the regulation of the coagulation cascade, acting as a serine protease inhibitor that specifically targets thrombin and factors IXa, Xa, and XIa, thereby preventing excessive blood clotting. ATIII enhances inhibitory activity in the presence of heparin, a naturally occurring anticoagulant. This regulation is crucial for maintaining hemostatic balance, and deficiencies in the SERPINC1 gene, which encodes ATIII, can lead to ATIII deficiency, an autosomal dominant condition that increases the risk of thrombophilia. Understanding ATIII function is essential for developing therapeutic strategies for clotting disorders and improving patient outcomes in conditions related to abnormal coagulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ATIII Antibody (H-7) References:

1. Familial overexpression of beta antithrombin caused by an Asn135Thr substitution.  |  Bayston, TA., et al. 1999. Blood. 93: 4242-7. PMID: 10361121
2. Two novel gene mutations in type I antithrombin deficiency.  |  Niiya, K., et al. 2001. Int J Hematol. 74: 469-72. PMID: 11794707
3. Antithrombin III pretreatment reduces neutrophil recruitment into the lung and skeletal muscle tissues in the rat model of bilateral lower limb ischemia and reperfusion: a pilot study.  |  Duru, S., et al. 2005. Acta Anaesthesiol Scand. 49: 1142-8. PMID: 16095456
4. Transgenic antithrombin III (Genzyme).  |  Yeung, PK. 2000. IDrugs. 3: 669-73. PMID: 16096931
5. Clopidogrel has no effect on D-dimer and thrombin-antithrombin III levels in patients with peripheral arterial disease undergoing peripheral percutaneous transluminal angioplasty.  |  Cassar, K., et al. 2005. J Vasc Surg. 42: 252-8. PMID: 16102623
6. Serum Level of Antithrombin III (ATIII) Could Serve as a Prognostic Biomarker in Neonatal Sepsis.  |  Samra, N., et al. 2019. Fetal Pediatr Pathol. 38: 290-298. PMID: 30931675
7. Heparinase Digestion of 3-O-Sulfated Sequences: Selective Heparinase II Digestion for Separation and Identification of Binding Sequences Present in ATIII Affinity Fractions of Bovine Intestinal Heparins.  |  Mourier, P. 2022. Front Med (Lausanne). 9: 841726. PMID: 35433769
8. Building Block Analysis of ATIII Affinity Fractions of Heparins: Application to the ATIII Binding Capacity of Non-conventional 3-O-Sulfated Sequences.  |  Mourier, P. 2022. Front Med (Lausanne). 9: 841738. PMID: 35514744
9. Familial thrombosis: inherited deficiency of antithrombin III.  |  Mackie, M., et al. 1978. Br Med J. 1: 136-8. PMID: 620225
10. Transfusion studies in patients with familial antithrombin III (ATIII) deficiency: half-disappearance time of infused ATIII and influence of such infusion on platelet life-span.  |  Winter, JH., et al. 1981. Br J Haematol. 49: 449-53. PMID: 7295588
11. Hereditary antithrombin III deficiency in an English family.  |  Scully, MF., et al. 1981. Br J Haematol. 47: 235-40. PMID: 7470393
12. Antithrombin III Kumamoto II; a single mutation at Arg393-His increased the affinity of antithrombin III for heparin.  |  Okajima, K., et al. 1995. Am J Hematol. 48: 12-8. PMID: 7832187

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATIII Antibody (H-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ATIII Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATIII Antibody (H-7) Specifications

Alternate Product Name: ATIII

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 331-400

Epitope Target: ATIII

Epitope Species: human

Product Reactivity/Detection: ATIII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ATIII Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin