Atg12 Antibody (C-6): sc-271688
- Atg12 Antibody (C-6) is a mouse monoclonal IgG2a κ Atg12 antibody, cited in 30 publications, provided at 200 µg/ml
- raised against amino acids 1-140 representing full length Atg12 of human origin
- Atg12 Antibody (C-6) is recommended for detection of Atg12 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Atg12 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Atg12 Antibody (C-6) for WB applications. These reagents are now offered in bundles with Atg12 Antibody (C-6) (see ordering information below).
Atg12 Antibody (C-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Atg12 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Atg12 antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Atg12, also known as autophagy-related protein 12, is a 140 amino acid protein that plays a crucial role in the autophagy process, which is essential for cellular homeostasis and the degradation of damaged organelles and proteins. Atg12 is primarily located in the cytoplasm, where Atg12 forms a vital conjugate with APG5, a reaction facilitated by APG7 and Atg10 in the presence of ATP. This conjugation is critical for autophagosome formation, as Atg12 regulates membrane morphology and the overall autophagic process. Atg12′s role in autophagy underscores Atg12′s potential as a therapeutic target in diseases characterized by impaired autophagic function, such as neurodegenerative disorders and cancer. Additionally, alternative splicing of the Atg12 gene results in two isoforms, further highlighting Atg12′s complexity and regulatory mechanisms in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Atg12 Antibody (C-6) References:
- Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. | Mizushima, N., et al. 2001. J Cell Biol. 152: 657-68. PMID: 11266458
- Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. | Tanida, I., et al. 2002. J Biol Chem. 277: 13739-44. PMID: 11825910
- Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method. | Mizushima, N., et al. 2002. FEBS Lett. 532: 450-4. PMID: 12482611
- Nitric oxide differentially regulates the gene expression of caspase genes but not some autophagic genes. | Rabkin, SW. and Klassen, SS. 2007. Nitric Oxide. 16: 339-47. PMID: 17227718
- The Atg5 Atg12 conjugate associates with innate antiviral immune responses. | Jounai, N., et al. 2007. Proc Natl Acad Sci U S A. 104: 14050-5. PMID: 17709747
- ATG12-ATG3 connects basal autophagy and late endosome function. | Murrow, L. and Debnath, J. 2015. Autophagy. 11: 961-2. PMID: 25998418
- Atg12-Interacting Motif Is Crucial for E2-E3 Interaction in Plant Atg8 System. | Matoba, K. and Noda, NN. 2021. Biol Pharm Bull. 44: 1337-1343. PMID: 34193767
- LncRNA KCNQ1OT1 promotes Atg12-mediated autophagy via inhibiting miR-26a-5p in ischemia reperfusion. | Xu, X., et al. 2021. Int J Cardiol. 339: 132-133. PMID: 34265311
- An ATG12-ATG5-TECPR1 E3-like complex regulates unconventional LC3 lipidation at damaged lysosomes. | Corkery, DP., et al. 2023. EMBO Rep. 24: e56841. PMID: 37381828
- Cloning and tissue expression of cDNAs from chromosome 5q21-22 which is frequently deleted in advanced lung cancer. | Ueno, K., et al. 1998. Hum Genet. 102: 63-8. PMID: 9490301
- A protein conjugation system essential for autophagy. | Mizushima, N., et al. 1998. Nature. 395: 395-8. PMID: 9759731
- A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. | Mizushima, N., et al. 1998. J Biol Chem. 273: 33889-92. PMID: 9852036
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Atg12 Antibody (C-6) | sc-271688 | 200 µg/ml | $322.00 | |||
Atg12 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-529243 | 200 µg Ab; 10 µg BP | $361.00 | |||
Atg12 Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-522025 | 200 µg Ab, 40 µg BP | $361.00 | |||
Atg12 Antibody (C-6) AC | sc-271688 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Atg12 Antibody (C-6) HRP | sc-271688 HRP | 200 µg/ml | $322.00 | |||
Atg12 Antibody (C-6) FITC | sc-271688 FITC | 200 µg/ml | $336.00 | |||
Atg12 Antibody (C-6) PE | sc-271688 PE | 200 µg/ml | $349.00 | |||
Atg12 Antibody (C-6) Alexa Fluor® 488 | sc-271688 AF488 | 200 µg/ml | $364.00 | |||
Atg12 Antibody (C-6) Alexa Fluor® 546 | sc-271688 AF546 | 200 µg/ml | $364.00 | |||
Atg12 Antibody (C-6) Alexa Fluor® 594 | sc-271688 AF594 | 200 µg/ml | $364.00 | |||
Atg12 Antibody (C-6) Alexa Fluor® 647 | sc-271688 AF647 | 200 µg/ml | $364.00 | |||
Atg12 Antibody (C-6) Alexa Fluor® 680 | sc-271688 AF680 | 200 µg/ml | $364.00 | |||
Atg12 Antibody (C-6) Alexa Fluor® 790 | sc-271688 AF790 | 200 µg/ml | $364.00 |