Anti-ATF-1/ATF1 Antibody (25C10G) is a mouse monoclonal IgG1 κ ATF-1/ATF1 antibody, cited in 101 publications, provided at 200 µg/ml
raised against amino acids 39-271 mapping within the DNA binding and dimerization domain of ATF-1 of human origin
Anti-ATF-1/ATF1 Antibody (25C10G) is recommended for detection of ATF-1 p35, CREB-1 p43 and CREM-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
Anti-ATF-1/ATF1 Antibody (25C10G) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-270 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-1/ATF1 (25C10G): sc-270.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ATF-1/ATF1 Antibody (25C10G) for WB and IHC(P) applications. These reagents are now offered in bundles with ATF-1/ATF1 Antibody (25C10G) (see ordering information below).
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Labware ships FedEx Ground free of charge to the contiguous US
ATF-1 Antibody (25C10G) is a high quality monoclonal ATF-1 antibody (also designated ATF1 antibody, activating transcription factor 1 antibody or TREB36 antibody) suitable for the detection of the ATF-1 protein of mouse, rat and human origin. ATF-1 Antibody (25C10G) is available as both the non-conjugated anti-ATF-1 antibody form, as well as multiple conjugated forms of anti-ATF-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1. ATF-1 binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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