ATF-1/ATF1 Antibody (25C10G): sc-270

ATF-1 Antibody (25C10G) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATF-1 of mouse, rat, and human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). ATF-1 (25C10G) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. ATF-1 plays a crucial role in regulating gene transcription, particularly in response to cellular stress and signaling pathways. ATF-1 is a member of the ATF/CREB transcription factor family, which is essential for binding to the cAMP response element (CRE) and facilitating the transcription of target genes involved in various physiological processes. ATF-1 mediates the induction of NOX1, highlighting ATF-1′s importance in reactive oxygen species production, which is vital for cellular signaling and homeostasis. ATF-1 interacts with other transcription factors, such as ATF-2 and c-Jun, to form complexes that initiate CRE-dependent transcription, thereby influencing a wide range of biological responses. Phosphorylation of ATF-1 at specific residues is critical for transcriptional activity, underscoring the significance of post-translational modifications in regulating function. ATF-1 (25C10G) antibody serves as a valuable tool for studying the intricate mechanisms of gene regulation and cellular responses mediated by ATF-1 in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ATF-1/ATF1 Antibody (25C10G) References:

1. ATF-1 is activated in response to UV irradiation in B16 melanoma cells.  |  Moore, BA., et al. 1999. Mol Cell Biol Res Commun. 1: 1-6. PMID: 10329470
2. ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation.  |  Kawasaki, H., et al. 2000. Nature. 405: 195-200. PMID: 10821277
3. Targeting the ATF-1/CREB transcription factors by single chain Fv fragment in human melanoma: potential modality for cancer therapy.  |  Jean, D. and Bar-Eli, M. 2001. Crit Rev Immunol. 21: 275-86. PMID: 11642609
4. Crosstalk between Myc and activating transcription factor 2 (ATF2): Myc prolongs the half-life and induces phosphorylation of ATF2.  |  Miethe, J., et al. 2001. Oncogene. 20: 8116-24. PMID: 11781825
5. Transactivation of the EGF receptor and a PI3 kinase-ATF-1 pathway is involved in the upregulation of NOX1, a catalytic subunit of NADPH oxidase.  |  Fan, C., et al. 2005. FEBS Lett. 579: 1301-5. PMID: 15710429
6. Assignment of the gene for cyclic AMP-response element binding protein 2 (CREB2) to human chromosome 2q24.1-q32.  |  Diep, A., et al. 1991. Genomics. 11: 1161-3. PMID: 1838349
7. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
8. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
9. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
10. MC1R signaling through the cAMP-CREB/ATF-1 and ERK-NFκB pathways accelerates G1/S transition promoting breast cancer progression.  |  Chelakkot, VS., et al. 2023. NPJ Precis Oncol. 7: 85. PMID: 37679505
11. ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents.  |  van Dam, H., et al. 1995. EMBO J. 14: 1798-811. PMID: 7737130
12. Heterodimer formation of cJun and ATF-2 is responsible for induction of c-jun by the 243 amino acid adenovirus E1A protein.  |  van Dam, H., et al. 1993. EMBO J. 12: 479-87. PMID: 8382609

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATF-1/ATF1 Antibody (25C10G) has 100 citations in a variety of scientific publications.

Primary antibodies like Anti-ATF-1/ATF1 Antibody (25C10G) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATF-1/ATF1 Antibody (25C10G) Specifications

Alternate Product Name: ATF1

Product Clone Name: 25C10G

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 39-271

Epitope Target: ATF-1

Epitope Species: human

Product Reactivity/Detection: ATF-1 p35, CREB-1 p43 and CREM-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 100 citations in various scientific publications

Available Conjugates/Forms for ATF-1/ATF1 Antibody (25C10G): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred CREB1 antibody is CREB1 Antibody (D-12): sc-377154.