ATAD3A/B/C Antibody (A-4): sc-376185
- ATAD3A/B/C Antibody (A-4) is a mouse monoclonal IgG1 κ ATAD3A/B/C antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 407-634 mapping at the C-terminus of ATAD3A of human origin
- ATAD3A/B/C Antibody (A-4) is recommended for detection of ATAD3A, ATAD3B and ATAD3C of human origin, and ATAD3A of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ATAD3A/B/C Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ATAD3A/B/C Antibody (A-4) for WB and IHC(P) applications. These reagents are now offered in bundles with ATAD3A/B/C Antibody (A-4) (see ordering information below).
ATAD3A/B/C Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATAD3A, ATAD3B, and ATAD3C proteins of human origin, as well as ATAD3A protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ATAD3A/B/C antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATAD3A protein plays a crucial role in mitochondrial function, particularly in stabilizing mitochondrial nucleoids, which are essential for organizing and maintaining mitochondrial DNA. These nucleoids are large complexes consisting of mtDNA and associated proteins, and their stability is vital for proper mitochondrial replication and transcription. Disruption of ATAD3A function can lead to mitochondrial dysfunction, implicated in various diseases, including neurodegenerative disorders and certain types of cancer. ATAD3A, ATAD3B, and ATAD3C genes are located on human chromosome 1, a region encompassing over 3,000 genes and representing approximately 8% of the human genome, highlighting these proteins′ importance in cellular processes and potential as therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ATAD3A/B/C Antibody (A-4) References:
- AAA+ superfamily ATPases: common structure--diverse function. | Ogura, T. and Wilkinson, AJ. 2001. Genes Cells. 6: 575-97. PMID: 11473577
- The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol. | Ye, Y., et al. 2001. Nature. 414: 652-6. PMID: 11740563
- Evolutionary history and higher order classification of AAA+ ATPases. | Iyer, LM., et al. 2004. J Struct Biol. 146: 11-31. PMID: 15037234
- New insights into the evolution of chromosome 1. | Weise, A., et al. 2005. Cytogenet Genome Res. 108: 217-22. PMID: 15545733
- [Structure, function and mechanisms of action of ATPases from the AAA superfamily of proteins]. | Kedzierska, S. 2006. Postepy Biochem. 52: 330-8. PMID: 17201069
- The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization. | He, J., et al. 2007. J Cell Biol. 176: 141-6. PMID: 17210950
- ATAD 3A and ATAD 3B are distal 1p-located genes differentially expressed in human glioma cell lines and present in vitro anti-oncogenic and chemoresistant properties. | Hubstenberger, A., et al. 2008. Exp Cell Res. 314: 2870-83. PMID: 18639545
- ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA. | Shu, L., et al. 2021. EMBO J. 40: e106283. PMID: 33665835
- ATAD3A: A Key Regulator of Mitochondria-Associated Diseases. | Chen, L., et al. 2023. Int J Mol Sci. 24: PMID: 37569886
- 'ATAD3C regulates ATAD3A assembly and function in the mitochondrial membrane'. | Gaudó, P., et al. 2024. Free Radic Biol Med. 211: 114-126. PMID: 38092275
- The AAA team: related ATPases with diverse functions. | Patel, S. and Latterich, M. 1998. Trends Cell Biol. 8: 65-71. PMID: 9695811
- AAA+: A class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes. | Neuwald, AF., et al. 1999. Genome Res. 9: 27-43. PMID: 9927482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATAD3A/B/C Antibody (A-4) | sc-376185 | 200 µg/ml | $322.00 | |||
ATAD3A/B/C Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-529831 | 200 µg Ab; 10 µg BP | $361.00 | |||
ATAD3A/B/C Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-522866 | 200 µg Ab, 40 µg BP | $361.00 | |||
ATAD3A/B/C Antibody (A-4) AC | sc-376185 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ATAD3A/B/C Antibody (A-4) HRP | sc-376185 HRP | 200 µg/ml | $322.00 | |||
ATAD3A/B/C Antibody (A-4) FITC | sc-376185 FITC | 200 µg/ml | $336.00 | |||
ATAD3A/B/C Antibody (A-4) PE | sc-376185 PE | 200 µg/ml | $349.00 | |||
ATAD3A/B/C Antibody (A-4) Alexa Fluor® 488 | sc-376185 AF488 | 200 µg/ml | $364.00 | |||
ATAD3A/B/C Antibody (A-4) Alexa Fluor® 546 | sc-376185 AF546 | 200 µg/ml | $364.00 | |||
ATAD3A/B/C Antibody (A-4) Alexa Fluor® 594 | sc-376185 AF594 | 200 µg/ml | $364.00 | |||
ATAD3A/B/C Antibody (A-4) Alexa Fluor® 647 | sc-376185 AF647 | 200 µg/ml | $364.00 | |||
ATAD3A/B/C Antibody (A-4) Alexa Fluor® 680 | sc-376185 AF680 | 200 µg/ml | $364.00 | |||
ATAD3A/B/C Antibody (A-4) Alexa Fluor® 790 | sc-376185 AF790 | 200 µg/ml | $364.00 |