ASPH Antibody (A-10): sc-271391

ASPH Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects ASPH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-ASPH antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Aspartyl/asparaginyl beta-hydroxylase (ASPH) is a crucial type II membrane protein primarily located in the endoplasmic reticulum, where ASPH plays a significant role in calcium homeostasis. ASPH is responsible for hydroxylating aspartate or asparagine residues in the epidermal growth factor-like (EGF) domains of several target proteins, utilizing iron as a cofactor in this enzymatic process. This post-translational modification is vital for proper functioning of these proteins, influencing their stability, activity, and interactions with other cellular components. ASPH gene encodes three distinct proteins—ASPH, Junctin, and Junctate (or Humbug)—which exhibit significant variation in their C-terminal domains and are expressed as five transcript variants. These variants differ in their roles related to calcium storage and release, hydroxylation capabilities, and tissue specificity, with all variants being present in skeletal muscle, while only some are found in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. In the endoplasmic reticulum lumen, ASPH can be processed into two different forms, further underscoring functional diversity and importance in cellular processes.

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ASPH Antibody (A-10) References:

1. Absence of post-translational aspartyl beta-hydroxylation of epidermal growth factor domains in mice leads to developmental defects and an increased incidence of intestinal neoplasia.  |  Dinchuk, JE., et al. 2002. J Biol Chem. 277: 12970-7. PMID: 11773073
2. Myocyte enhancer factor 2 activates promoter sequences of the human AbetaH-J-J locus, encoding aspartyl-beta-hydroxylase, junctin, and junctate.  |  Feriotto, G., et al. 2005. Mol Cell Biol. 25: 3261-75. PMID: 15798210
3. The assembly of calcium release units in cardiac muscle.  |  Franzini-Armstrong, C., et al. 2005. Ann N Y Acad Sci. 1047: 76-85. PMID: 16093486
4. Overexpression of junctin causes adaptive changes in cardiac myocyte Ca(2+) signaling.  |  Kirchhefer, U., et al. 2006. Cell Calcium. 39: 131-42. PMID: 16289269
5. ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis.  |  Lin, Q., et al. 2019. Mol Cancer. 18: 156. PMID: 31694640
6. ASPH Regulates Osteogenic Differentiation and Cellular Senescence of BMSCs.  |  Peng, H., et al. 2020. Front Cell Dev Biol. 8: 872. PMID: 33015050
7. Upregulation of circ-ASPH contributes to glioma cell proliferation and aggressiveness by targeting the miR-599/AR/SOCS2-AS1 signaling pathway.  |  Qu, Y., et al. 2021. Oncol Lett. 21: 388. PMID: 33777211
8. Aspartate β-Hydroxylase (ASPH) Expression in Acute Myeloid Leukemia: A Potential Novel Therapeutic Target.  |  Holtzman, NG., et al. 2021. Front Oncol. 11: 783744. PMID: 35004304
9. Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility.  |  Endo, Y., et al. 2022. Nat Commun. 13: 3403. PMID: 35697689
10. Aspartate β-hydroxylase (ASPH) Accelerates Intrahepatic Cholangiocarcinoma Metastasis via Upregulating SHH Signaling Pathway.  |  Zou, Y., et al. 2023. Curr Protein Pept Sci. 24: 436-446. PMID: 37132101
11. ASPH dysregulates cell death and induces chemoresistance in hepatocellular carcinoma.  |  Li, J., et al. 2024. Cancer Lett. 611: 217396. PMID: 39706251
12. Cloning and characterization of the human gene encoding aspartyl beta-hydroxylase.  |  Korioth, F., et al. 1994. Gene. 150: 395-9. PMID: 7821814

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ASPH Antibody (A-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ASPH Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ASPH Antibody (A-10) Specifications

Alternate Product Name: ASPH

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 382-681

Epitope Target: ASPH

Epitope Species: human

Product Reactivity/Detection: ASPH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ASPH Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin