ASF1B Antibody (C-6): sc-393169
- ASF1B Antibody (C-6) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 1-202 representing full length ASF1B of human origin
- Anti-ASF1B Antibody (C-6) is recommended for detection of ASF1B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ASF1B Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ASF1B Antibody (C-6) for WB applications. These reagents are now offered in bundles with ASF1B Antibody (C-6) (see ordering information below).
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ASF1B Antibody (C-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects ASF1B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ASF1B (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASF1B protein plays a crucial role in gene expression regulation by acting as a histone chaperone, facilitating nucleosome assembly and disassembly, which is essential for DNA replication and repair. This function ensures proper chromatin remodeling, allowing DNA access for transcription and other cellular processes. ASF1B gene is located on chromosome 19p13.12, and ASF1B interaction with core histones H3 and H4 is vital for maintaining chromatin structure and function. ASF1B also cooperates with CAF1 protein in nucleosome formation, highlighting ASF1B significance in epigenetic regulation of gene expression. With detection capabilities across multiple species and applications, anti-ASF1B antibody (C-6) serves as an invaluable tool for researchers studying chromatin dynamics and gene regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ASF1B Antibody (C-6) References:
- Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II. | Schwabish, MA. and Struhl, K. 2006. Mol Cell. 22: 415-22. PMID: 16678113
- TLK1B promotes repair of UV-damaged DNA through chromatin remodeling by Asf1. | Sen, SP. and De Benedetti, A. 2006. BMC Mol Biol. 7: 37. PMID: 17054786
- Asf1, a loveseat for a histone couple. | Bao, Y. and Shen, X. 2006. Cell. 127: 458-60. PMID: 17081967
- Structural basis for the histone chaperone activity of Asf1. | English, CM., et al. 2006. Cell. 127: 495-508. PMID: 17081973
- Structure of the yeast histone H3-ASF1 interaction: implications for chaperone mechanism, species-specific interactions, and epigenetics. | Antczak, AJ., et al. 2006. BMC Struct Biol. 6: 26. PMID: 17166288
- The histone chaperone Asf1 at the crossroads of chromatin and DNA checkpoint pathways. | Mousson, F., et al. 2007. Chromosoma. 116: 79-93. PMID: 17180700
- Structure of the histone chaperone ASF1 bound to the histone H3 C-terminal helix and functional insights. | Agez, M., et al. 2007. Structure. 15: 191-9. PMID: 17292837
- Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4. | Natsume, R., et al. 2007. Nature. 446: 338-41. PMID: 17293877
- Transcriptional regulation of human chromatin assembly factor ASF1. | Hayashi, R., et al. 2007. DNA Cell Biol. 26: 91-9. PMID: 17328667
- RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity. | Feng, S., et al. 2022. Nat Commun. 13: 957. PMID: 35177609
- The TLK-ASF1 histone chaperone pathway plays a critical role in IL-1β-mediated AML progression. | Lin, HY., et al. 2024. Blood. 143: 2749-2762. PMID: 38498025
- Mechanism of ASF1 Inhibition by CDAN1. | Sedor, SF. and Shao, S. 2024. bioRxiv.. PMID: 39149339
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASF1B Antibody (C-6) | sc-393169 | 200 µg/ml | $322.00 | |||
ASF1B Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-530379 | 200 µg Ab; 10 µg BP | $361.00 | |||
ASF1B Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-523835 | 200 µg Ab, 40 µg BP | $361.00 | |||
ASF1B Antibody (C-6): m-IgG2b BP-HRP Bundle | sc-550183 | 200 µg Ab; 10 µg BP | $361.00 | |||
ASF1B Antibody (C-6) AC | sc-393169 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ASF1B Antibody (C-6) HRP | sc-393169 HRP | 200 µg/ml | $322.00 | |||
ASF1B Antibody (C-6) FITC | sc-393169 FITC | 200 µg/ml | $336.00 | |||
ASF1B Antibody (C-6) PE | sc-393169 PE | 200 µg/ml | $349.00 | |||
ASF1B Antibody (C-6) Alexa Fluor® 488 | sc-393169 AF488 | 200 µg/ml | $364.00 | |||
ASF1B Antibody (C-6) Alexa Fluor® 546 | sc-393169 AF546 | 200 µg/ml | $364.00 | |||
ASF1B Antibody (C-6) Alexa Fluor® 594 | sc-393169 AF594 | 200 µg/ml | $364.00 | |||
ASF1B Antibody (C-6) Alexa Fluor® 647 | sc-393169 AF647 | 200 µg/ml | $364.00 | |||
ASF1B Antibody (C-6) Alexa Fluor® 680 | sc-393169 AF680 | 200 µg/ml | $364.00 | |||
ASF1B Antibody (C-6) Alexa Fluor® 790 | sc-393169 AF790 | 200 µg/ml | $364.00 |