ASC-1 Antibody (F-7): sc-376916

ASC-1 Antibody (F-7) is a mouse monoclonal IgG1 antibody that detects Activating Signal Co-integrator-1 (ASC-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ASC-1, originally identified as TRIP4, serves as a crucial transcriptional co-activator of nuclear receptors, playing a significant role in gene expression regulation by associating with components of the RNA polymerase II complex and binding to essential basal transcription factors like TBP and TFIIA. ASC-1′s structure includes a zinc finger motif that facilitates interaction with other transcription integrators, such as SRC-1 and CBP/p300, which are vital for ligand-dependent transactivation capabilities. ASC-1 is predominantly localized in the nucleus of HeLa cells, but under serum starvation conditions, can translocate to the cytoplasm. This dynamic localization suggests ASC-1 may help establish distinct co-activator complexes depending on the cellular environment, thereby influencing transcriptional outcomes in response to various stimuli. ASC-1′s versatility in different cellular contexts underscores its importance in regulating gene expression and maintaining cellular homeostasis, making anti-ASC-1 antibody (F-7) an essential tool for researchers studying transcriptional regulation and co-activator dynamics.

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ASC-1 Antibody (F-7) References:

1. Modification of ASC1 by UFM1 is crucial for ERα transactivation and breast cancer development.  |  Yoo, HM., et al. 2014. Mol Cell. 56: 261-274. PMID: 25219498
2. The Asc-1 Complex Disassembles Collided Ribosomes.  |  Juszkiewicz, S., et al. 2020. Mol Cell. 79: 603-614.e8. PMID: 32579943
3. Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population.  |  Suwandhi, L., et al. 2021. Nat Commun. 12: 1588. PMID: 33707431
4. Inherited Defects of the Asc-1 Complex in Congenital Neuromuscular Diseases.  |  Meunier, J., et al. 2021. Int J Mol Sci. 22: PMID: 34204919
5. Synergistic Control of Transmitter Turnover at Glycinergic Synapses by GlyT1, GlyT2, and Asc-1.  |  Eulenburg, V. and Hülsmann, S. 2022. Int J Mol Sci. 23: PMID: 35269698
6. Further clinical and genetic evidence of Asc-1 complex dysfunction in congenital neuromuscular disease.  |  Marais, A., et al. 2022. Eur J Med Genet. 65: 104537. PMID: 35690317
7. The Asc-1 complex promotes translation initiation by scanning ribosomes.  |  Kito, Y., et al. 2023. EMBO J. 42: e112869. PMID: 37092320
8. Dual Role of Dysfunctional Asc-1 Transporter in Distinct Human Pathologies, Human Startle Disease, and Developmental Delay.  |  Drehmann, P., et al. 2023. eNeuro. 10: PMID: 37903619
9. Structure and mechanisms of transport of human Asc1/CD98hc amino acid transporter.  |  Rullo-Tubau, J., et al. 2024. Nat Commun. 15: 2986. PMID: 38582862
10. Cryo-EM structure of the human Asc-1 transporter complex.  |  Li, Y., et al. 2024. Nat Commun. 15: 3036. PMID: 38589439

The preferred ASC-1 antibody is ASC-1 Antibody (F-8): sc-365202.