ARP-1 Antibody (B-2): sc-271265

ARP-1 Antibody (B-2) is a mouse monoclonal IgG1 antibody that detects ARP-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARP-1, also known as COUP-TFII, plays a crucial role in various biological processes, particularly in gene expression regulation during development. ARP-1 is primarily located in the nucleus, where ARP-1 functions as a transcription factor, influencing the expression of genes involved in cellular differentiation and development. Nuclear localization of ARP-1 enables interaction with specific DNA response elements, thereby modulating target gene transcription. ARP-1 interacts with other transcription factors, such as retinoic acid receptors and peroxisome proliferator-activated receptors, which underscores ARP-1′s importance in coordinating complex gene regulatory networks. ARP-1′s ability to act as both a negative regulator and transcription initiator highlights ARP-1′s versatile role in cellular processes, making ARP-1 a significant focus of research in developmental biology and disease mechanisms.

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ARP-1 Antibody (B-2) References:

1. Orphan receptor Arp-1 binds to the nucleotide sequence located between TATA box and transcriptional initiation site of the human angiotensinogen gene and reduces estrogen induced promoter activity.  |  Narayanan, CS., et al. 1999. Mol Cell Endocrinol. 148: 79-86. PMID: 10221773
2. Matrix-mediated changes in the expression of HNF-4alpha isoforms and in DNA-binding activity of ARP-1 in primary cultures of rat hepatocytes.  |  Runge, D., et al. 1999. Biochem Biophys Res Commun. 259: 651-5. PMID: 10364473
3. Transcriptional regulation of the nuclear gene encoding the alpha-subunit of the mammalian mitochondrial F1F0 ATP synthase complex: role for the orphan nuclear receptor, COUP-TFII/ARP-1.  |  Jordan, EM., et al. 2003. Biochemistry. 42: 2656-63. PMID: 12614160
4. ARP-1/COUP-TF II determines hepatoma phenotype by acting as both a transcriptional repressor of microsomal triglyceride transfer protein and an inducer of CYP7A1.  |  Kang, S., et al. 2003. J Biol Chem. 278: 30478-86. PMID: 12777384
5. Repression by ARP-1 sensitizes apolipoprotein AI gene responsiveness to RXR alpha and retinoic acid.  |  Widom, RL., et al. 1992. Mol Cell Biol. 12: 3380-9. PMID: 1321332
6. Transcriptional regulation of human apolipoprotein genes ApoB, ApoCIII, and ApoAII by members of the steroid hormone receptor superfamily HNF-4, ARP-1, EAR-2, and EAR-3.  |  Ladias, JA., et al. 1992. J Biol Chem. 267: 15849-60. PMID: 1639815
7. ARP-1 Regulates the Transcriptional Activity of the Aromatase Gene in the Mouse Brain.  |  Honda, SI. and Harada, N. 2020. Front Endocrinol (Lausanne). 11: 306. PMID: 32582022
8. Interaction of the peroxisome proliferator-activated receptor alpha with the retinoid X receptor alpha unmasks a cryptic peroxisome proliferator response element that overlaps an ARP-1-binding site in the CYP4A6 promoter.  |  Palmer, CN., et al. 1994. J Biol Chem. 269: 18083-9. PMID: 8027069
9. The directly repeated RG(G/T)TCA motifs of the rat and mouse cellular retinol-binding protein II genes are promiscuous binding sites for RAR, RXR, HNF-4, and ARP-1 homo- and heterodimers.  |  Nakshatri, H. and Chambon, P. 1994. J Biol Chem. 269: 890-902. PMID: 8288643
10. Multiple parameters determine the specificity of transcriptional response by nuclear receptors HNF-4, ARP-1, PPAR, RAR and RXR through common response elements.  |  Nakshatri, H. and Bhat-Nakshatri, P. 1998. Nucleic Acids Res. 26: 2491-9. PMID: 9580705

The preferred COUP-TFI antibody is COUP-TFI Antibody (H-11): sc-74561.