COUP-TFI Antibody (H-11): sc-74561

COUP-TFI Antibody (H-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects COUP-TFI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-COUP-TFI antibody (H-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COUP-TFI protein, also known as chicken ovalbumin upstream promoter transcription factor I, plays a crucial role in regulating gene expression during development, particularly in the central nervous system. COUP-TFI′s ability to bind to specific response elements allows modulation of other transcription factors′ activity, such as retinoic acid receptors and peroxisome proliferator-activated receptors, thereby influencing processes like neural differentiation and development. COUP-TFI expression is tightly regulated, and COUP-TFI′s interaction with other proteins in the steroid/thyroid hormone receptor superfamily highlights COUP-TFI′s importance in maintaining cellular homeostasis and responding to developmental cues. Using COUP-TFI monoclonal antibody (H-11) in research can provide valuable insights into regulatory mechanisms governing neural development and broader implications of transcription factor interactions in various biological processes.

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COUP-TFI Antibody (H-11) References:

1. Dynamic expression of COUP-TFI and COUP-TFII during development and functional maturation of the mouse inner ear.  |  Tang, LS., et al. 2005. Gene Expr Patterns. 5: 587-92. PMID: 15907456
2. Direct repeats as selective response elements for the thyroid hormone, retinoic acid, and vitamin D3 receptors.  |  Umesono, K., et al. 1991. Cell. 65: 1255-66. PMID: 1648450
3. COUP-TFI controls Notch regulation of hair cell and support cell differentiation.  |  Tang, LS., et al. 2006. Development. 133: 3683-93. PMID: 16914494
4. COUP-TFI is required for the formation of commissural projections in the forebrain by regulating axonal growth.  |  Armentano, M., et al. 2006. Development. 133: 4151-62. PMID: 17021036
5. Regulation of the apolipoprotein AI gene by ARP-1, a novel member of the steroid receptor superfamily.  |  Ladias, JA. and Karathanasis, SK. 1991. Science. 251: 561-5. PMID: 1899293
6. COUP transcription factor is a member of the steroid receptor superfamily.  |  Wang, LH., et al. 1989. Nature. 340: 163-6. PMID: 2739739
7. Identification of two novel members of erbA superfamily by molecular cloning: the gene products of the two are highly related to each other.  |  Miyajima, N., et al. 1988. Nucleic Acids Res. 16: 11057-74. PMID: 2905047
8. COUP-TFI specifies the medial entorhinal cortex identity and induces differential cell adhesion to determine the integrity of its boundary with neocortex.  |  Feng, J., et al. 2021. Sci Adv. 7: PMID: 34215582
9. An evolutionary conserved COUP-TF binding element in a neural-specific gene and COUP-TF expression patterns support a major role for COUP-TF in neural development.  |  Lu, XP., et al. 1994. Mol Endocrinol. 8: 1774-88. PMID: 7708064
10. Cloning and expression during development of three murine members of the COUP family of nuclear orphan receptors.  |  Jonk, LJ., et al. 1994. Mech Dev. 47: 81-97. PMID: 7947324
11. Multiple mechanisms of chicken ovalbumin upstream promoter transcription factor-dependent repression of transactivation by the vitamin D, thyroid hormone, and retinoic acid receptors.  |  Cooney, AJ., et al. 1993. J Biol Chem. 268: 4152-60. PMID: 8382695
12. Chicken ovalbumin upstream promoter-transcription factors and their regulation.  |  Qiu, Y., et al. 1996. J Steroid Biochem Mol Biol. 56: 81-5. PMID: 8603050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-COUP-TFI Antibody (H-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-COUP-TFI Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

COUP-TFI Antibody (H-11) Specifications

Alternate Product Name: COUP-TFI

Product Clone Name: H-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 261-320

Epitope Target: COUP-TFI

Epitope Species: human

Product Reactivity/Detection: COUP-TFI and ARP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for COUP-TFI Antibody (H-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent