Date published: 2025-10-19

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ARK-1 Antibody (C-1): sc-398814

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Datasheets
  • ARK-1 Antibody (C-1) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 1-130 mapping at the N-terminus of ARK-1 of human origin
  • ARK-1 Antibody (C-1) is recommended for detection of ARK-1 of human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-ARK-1 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ARK-1 Antibody (C-1) for WB and IHC(P) applications. These reagents are now offered in bundles with ARK-1 Antibody (C-1) (see ordering information below).

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ARK-1 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARK-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ARK-1 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Aurora related kinase-1 (ARK-1), also known as STK15, Aurora2, and Aik1, is a centrosome-associated serine/threonine kinase that plays a crucial role in regulating centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. Proper functioning of ARK-1 is vital for accurate cell division, as dysregulation can lead to aneuploidy and cancer. ARK-1 is predominantly localized in the nucleus and associates with distinct components of the mitotic machinery, particularly at the spindle poles during mitosis. ARK-1 protein levels are elevated in various colon carcinoma cell lines, such as HT-29 and SW480, and accumulates during metaphase in HeLa cells, highlighting potential as a biomarker for tumor progression. ARK-1 undergoes degradation after the G2/M phase via the ubiquitin-proteasome pathway, which is essential for maintaining proper cell cycle regulation. Expression of ARK-1 is particularly high in the human thymus and fetal liver, indicating importance in developmental processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

ARK-1 Antibody (C-1) References:

  1. Identification of Novel Aurora Kinase A (AURKA) Inhibitors via Hierarchical Ligand-Based Virtual Screening.  |  Kong, Y., et al. 2018. J Chem Inf Model. 58: 36-47. PMID: 29202231
  2. Aurora kinase A (AURKA) promotes the progression and imatinib resistance of advanced gastrointestinal stromal tumors.  |  Cheng, X., et al. 2021. Cancer Cell Int. 21: 407. PMID: 34332577
  3. Aurora kinase A regulates liver regeneration through macrophages polarization and Wnt/β-catenin signalling.  |  Yin, Y., et al. 2022. Liver Int. 42: 468-478. PMID: 34719108
  4. Aurora Kinase A as a Diagnostic and Prognostic Marker of Malignant Mesothelioma.  |  Guo, Z., et al. 2021. Front Oncol. 11: 789244. PMID: 34956905
  5. Targeting aurora kinase a (AURKA) in cancer: molecular docking and dynamic simulations of potential AURKA inhibitors.  |  Almilaibary, A. 2022. Med Oncol. 39: 246. PMID: 36180808
  6. The feedback loop of AURKA/DDX5/TMEM147-AS1/let-7 drives lipophagy to induce cisplatin resistance in epithelial ovarian cancer.  |  Shao, Y., et al. 2023. Cancer Lett. 565: 216241. PMID: 37217070
  7. Aurora kinase A regulates cancer-associated RNA aberrant splicing in breast cancer.  |  Li, S., et al. 2023. Heliyon. 9: e17386. PMID: 37415951
  8. eEF1A2 promotes PTEN-GSK3β-SCF complex-dependent degradation of Aurora kinase A and is inactivated in breast cancer.  |  Treekitkarnmongkol, W., et al. 2024. Sci Signal. 17: eadh4475. PMID: 38442201
  9. TonEBP inhibits ciliogenesis by controlling aurora kinase A and regulating centriolar satellite integrity.  |  Chinbold, B., et al. 2024. Cell Commun Signal. 22: 348. PMID: 38961488
  10. Caspase-mediated AURKA cleavage at Asp132 is essential for paclitaxel to elicit cell apoptosis.  |  Chen, X., et al. 2024. Theranostics. 14: 3909-3926. PMID: 38994036

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

ARK-1 Antibody (C-1)

sc-398814
200 µg/ml
$316.00

ARK-1 Antibody (C-1): m-IgG Fc BP-HRP Bundle

sc-530939
200 µg Ab; 10 µg BP
$354.00

ARK-1 Antibody (C-1): m-IgGκ BP-HRP Bundle

sc-524499
200 µg Ab, 40 µg BP
$354.00

ARK-1 Antibody (C-1): m-IgG1 BP-HRP Bundle

sc-544326
200 µg Ab; 20 µg BP
$354.00

ARK-1 Antibody (C-1) AC

sc-398814 AC
500 µg/ml, 25% agarose
$416.00

ARK-1 Antibody (C-1) HRP

sc-398814 HRP
200 µg/ml
$316.00

ARK-1 Antibody (C-1) FITC

sc-398814 FITC
200 µg/ml
$330.00

ARK-1 Antibody (C-1) PE

sc-398814 PE
200 µg/ml
$343.00

ARK-1 Antibody (C-1) Alexa Fluor® 488

sc-398814 AF488
200 µg/ml
$357.00

ARK-1 Antibody (C-1) Alexa Fluor® 546

sc-398814 AF546
200 µg/ml
$357.00

ARK-1 Antibody (C-1) Alexa Fluor® 594

sc-398814 AF594
200 µg/ml
$357.00

ARK-1 Antibody (C-1) Alexa Fluor® 647

sc-398814 AF647
200 µg/ml
$357.00

ARK-1 Antibody (C-1) Alexa Fluor® 680

sc-398814 AF680
200 µg/ml
$357.00

ARK-1 Antibody (C-1) Alexa Fluor® 790

sc-398814 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for Western blot for sc-398814: ARK-1 (C-1) monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from GoodARK-1 (C-1): sc-398814 The antibody was tested on whole lysate from liver cancer cell lines (JHH6 andHuh7) and immortalized hepatocytes (IHH). Antibody: show various bands which can represent shorter or longer forms of the protein. Apparent MW: about 22kDa, 46kDa, and 55kDa. Expected MW: 46kDa. • 40ug/lane cell lysate • Transfer 1h30 100V, PVDF (0.2 µm) • Blocking milk 4% in 0.1% Tween -PBS 1h RT • Anti- ARK-1 (C-1) (sc-398814):200 O/N 4°C in blocking solution • Anti-mouse HRP 1:1000 1h RT in blocking solution • Developed with ECL Plus
Date published: 2022-06-09
Rated 5 out of 5 by from Works very well!We bought this antibody a month ago. It is highly sensitive and shows no background.
Date published: 2018-07-27
Rated 5 out of 5 by from Works great!I used this antibody in a Western blot to validate an AURKA siRNA treatment with good results. It gives a clear band at ~50kD at 1:1000 dilution. There is also a nonspecific band at ~25kD.
Date published: 2017-09-13
Rated 5 out of 5 by from very good for ARK-1Very strong band was shown at a dilution of 1:800 in HepG2 cells.
Date published: 2017-07-06
Rated 5 out of 5 by from ImmunefluorescenceVery good results in IF staining of human adrenal and fibroblasts cells at a concentration of 1:50.
Date published: 2017-06-14
Rated 3 out of 5 by from Reasonably potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was detection of the ~46 kDa target in a HeLa/Jurkat cell lysate mix, but not in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for ~52,~ 34 and ~20 kDa proteins. A ~20 kDa medium cross-reactive protein was also evident in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Great Western blot data of ARKGreat Western blot data of ARK-1 expression in non-transfected 293 human ARK-1 transfected 293 HeLa and BT-20 whole cell lysates. -SCBT QC
Date published: 2014-01-05
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ARK-1 Antibody (C-1) is rated 4.6 out of 5 by 7.
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