ARK-1 Antibody (A-11): sc-514374

ARK-1 Antibody (A-11) is a mouse monoclonal IgG2a antibody that detects Aurora related kinase-1 (ARK-1) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARK-1 plays a crucial role in cell division by regulating centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis, making ARK-1 essential for maintaining genomic stability. ARK-1 is primarily localized in the nucleus and associates with key components of the mitotic machinery, particularly at the centrosome and spindle poles, where ARK-1 is vital for proper mitotic progression. Notably, ARK-1 expression is significantly elevated in various colon carcinoma cell lines, indicating ARK-1′s potential involvement in oncogenesis. Furthermore, ARK-1 protein levels are tightly regulated, as ARK-1 accumulates during metaphase and is subsequently degraded after the G2/M transition via the ubiquitin-proteasome pathway, highlighting the importance of precise regulation in the cell cycle. The unique genetic loci of ARK-1 relative to ARK-2 suggests these kinases may have distinct yet complementary roles in cell cycle dynamics, further underscoring ARK-1′s significance in cancer biology and cellular function.

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ARK-1 Antibody (A-11) References:

1. Aurora kinase A inhibition reverses the Warburg effect and elicits unique metabolic vulnerabilities in glioblastoma.  |  Nguyen, TTT., et al. 2021. Nat Commun. 12: 5203. PMID: 34471141
2. Aurora Kinase A Is Involved in Controlling the Localization of Aquaporin-2 in Renal Principal Cells.  |  Baltzer, S., et al. 2022. Int J Mol Sci. 23: PMID: 35054947
3. Unfolded Protein Response Is Activated by Aurora Kinase A in Esophageal Adenocarcinoma.  |  Lu, H., et al. 2022. Cancers (Basel). 14: PMID: 35326553
4. A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells.  |  Liu, F., et al. 2022. Adv Sci (Weinh). 9: e2104823. PMID: 35652200
5. AurkA nuclear localization is promoted by TPX2 and counteracted by protein degradation.  |  Asteriti, IA., et al. 2023. Life Sci Alliance. 6: PMID: 36797043
6. CDCA2 promotes melanoma progression by inhibiting ubiquitin-mediated degradation of Aurora kinase A.  |  Sun, W., et al. 2023. Eur J Cancer. 188: 49-63. PMID: 37196484
7. Aurora kinase A/AURKA functionally interacts with the mitochondrial ATP synthase to regulate energy metabolism and cell death.  |  Sharma, RK., et al. 2023. Cell Death Discov. 9: 203. PMID: 37386025
8. AURKA knockdown inhibits esophageal squamous cell carcinoma progression through ferroptosis.  |  Mi, Y., et al. 2024. Heliyon. 10: e28365. PMID: 38571661
9. The role of Aurora kinase A in hepatocellular carcinoma: Unveiling the intriguing functions of a key but still underexplored factor in liver cancer.  |  Grisetti, L., et al. 2024. Cell Prolif. 57: e13641. PMID: 38590119
10. Aurora kinase A inhibition plus Tumor Treating Fields suppress glioma cell proliferation in a cilium-independent manner.  |  Tian, J., et al. 2024. Transl Oncol. 45: 101956. PMID: 38640786

The preferred ARK-1 antibody is ARK-1 Antibody (35C1): sc-56881.