ARID1A Antibody (PSG3): sc-32761

ARID1A Antibody (PSG3) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARID1A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. ARID1A (PSG3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARID1A protein plays a crucial role in the SWI-SNF chromatin remodeling complex, which is essential for regulating gene expression through nucleosome structure alteration in an ATP-dependent manner. ARID1A′s ability to facilitate transcriptional activation is vital for numerous cellular processes, including differentiation and development, as ARID1A helps control DNA accessibility to transcription factors. The SWI-SNF complex comprises several key subunits, including Brm and Brg-1, which are ATPase components driving remodeling activity, while ARID1A, also known as BAF250A, has regulatory functions within the complex. ARID1A′s interaction with other subunits, such as Ini1, BAF155, and BAF170, enhances the remodeling capabilities of the complex, underscoring ARID1A′s importance in maintaining proper gene expression and cellular function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ARID1A Antibody (PSG3) References:

1. Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.  |  Phelan, ML., et al. 1999. Mol Cell. 3: 247-53. PMID: 10078207
2. The human SWI-SNF complex protein p270 is an ARID family member with non-sequence-specific DNA binding activity.  |  Dallas, PB., et al. 2000. Mol Cell Biol. 20: 3137-46. PMID: 10757798
3. Two related ARID family proteins are alternative subunits of human SWI/SNF complexes.  |  Wang, X., et al. 2004. Biochem J. 383: 319-25. PMID: 15170388
4. Expression of p270 (ARID1A), a component of human SWI/SNF complexes, in human tumors.  |  Wang, X., et al. 2004. Int J Cancer. 112: 636. PMID: 15382044
5. ARID1A Mutations Promote P300-Dependent Endometrial Invasion through Super-Enhancer Hyperacetylation.  |  Wilson, MR., et al. 2020. Cell Rep. 33: 108366. PMID: 33176148
6. Targeting ARID1A mutations in cancer.  |  Mullen, J., et al. 2021. Cancer Treat Rev. 100: 102287. PMID: 34619527
7. Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis.  |  Zhou, W., et al. 2023. Cancer Cell. 41: 740-756.e10. PMID: 36963401
8. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor.  |  Muchardt, C. and Yaniv, M. 1993. EMBO J. 12: 4279-90. PMID: 8223438
9. BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.  |  Khavari, PA., et al. 1993. Nature. 366: 170-4. PMID: 8232556
10. Nucleosome disruption by human SWI/SNF is maintained in the absence of continued ATP hydrolysis.  |  Imbalzano, AN., et al. 1996. J Biol Chem. 271: 20726-33. PMID: 8702824
11. p300/CREB binding protein-related protein p270 is a component of mammalian SWI/SNF complexes.  |  Dallas, PB., et al. 1998. Mol Cell Biol. 18: 3596-603. PMID: 9584200

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARID1A Antibody (PSG3) has 120 citations in a variety of scientific publications.

Primary antibodies like Anti-ARID1A Antibody (PSG3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARID1A Antibody (PSG3) Specifications

Alternate Product Name: ARID1A

Product Clone Name: PSG3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 600-1018

Epitope Target: ARID1A

Epitope Species: human

Product Reactivity/Detection: ARID1A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 120 citations in various scientific publications

Available Conjugates/Forms for ARID1A Antibody (PSG3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred ARID1A antibody is ARID1A Antibody (C-7): sc-373784.