ARHGAP4 Antibody (G-6): sc-376251
- ARHGAP4 Antibody (G-6) is a mouse monoclonal IgG1 κ ARHGAP4 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 101-190 mapping near the N-terminus of ARHGAP4 of human origin
- ARHGAP4 Antibody (G-6) is recommended for detection of ARHGAP4 of mouse, rat, human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ARHGAP4 Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for ARHGAP4 Antibody (G-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with ARHGAP4 Antibody (G-6) (see ordering information below).
ARHGAP4 Antibody (G-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARHGAP4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ARHGAP4 antibody (G-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARHGAP4, also known as Rho GTPase activating protein 4, RGC1, C1, p115, or RhoGAP4, is a cytoplasmic protein that plays a crucial role in regulating cell motility and axon outgrowth by modulating cytoskeletal dynamics. ARHGAP4 contains one Rho-GAP domain, one FCH (Fps/Fes/Fer/CIP4 homology) domain, and one SH3 (Src homology 3) domain, which are essential for inhibiting Rho GTPases such as Cdc42 and Rac1. These GTPases promote cell motility and axon outgrowth, making ARHGAP4 a significant player in cellular processes related to migration and development. ARHGAP4 is predominantly expressed in hematopoietic cells, with additional presence in lung, placenta, and certain fetal tissues, and localizes to the leading edge of migrating cells, axons, and growth cones, highlighting its importance in cellular movement and neural development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARHGAP4 Antibody (G-6) References:
- Compound deletion of the rhoGAP C1 and V2 vasopressin receptor genes in a patient with nephrogenic diabetes insipidus. | Schöneberg, T., et al. 1999. Hum Mutat. 14: 163-74. PMID: 10425039
- Two novel types of contiguous gene deletion of the AVPR2 and ARHGAP4 genes in unrelated Japanese kindreds with nephrogenic diabetes insipidus. | Demura, M., et al. 2002. Hum Mutat. 19: 23-9. PMID: 11754100
- p115 Rho GTPase activating protein interacts with MEKK1. | Christerson, LB., et al. 2002. J Cell Physiol. 192: 200-8. PMID: 12115726
- Cloning of rat ARHGAP4/C1, a RhoGAP family member expressed in the nervous system that colocalizes with the Golgi complex and microtubules. | Foletta, VC., et al. 2002. Brain Res Mol Brain Res. 107: 65-79. PMID: 12414125
- FNBP2 gene on human chromosome 1q32.1 encodes ARHGAP family protein with FCH, FBH, RhoGAP and SH3 domains. | Katoh, M. and Katoh, M. 2003. Int J Mol Med. 11: 791-7. PMID: 12736724
- Regulation of FasL expression: a SH3 domain containing protein family involved in the lysosomal association of FasL. | Qian, J., et al. 2006. Cell Signal. 18: 1327-37. PMID: 16318909
- Severe combined immunodeficiency associated with nephrogenic diabetes insipidus and a deletion in the Xq28 region. | Broides, A., et al. 2006. Clin Immunol. 120: 147-55. PMID: 16781893
- ARHGAP4 is a novel RhoGAP that mediates inhibition of cell motility and axon outgrowth. | Vogt, DL., et al. 2007. Mol Cell Neurosci. 36: 332-42. PMID: 17804252
- An X chromosome-linked gene encoding a protein with characteristics of a rhoGAP predominantly expressed in hematopoietic cells. | Tribioli, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 695-9. PMID: 8570618
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARHGAP4 Antibody (G-6) | sc-376251 | 200 µg/ml | $316.00 | |||
ARHGAP4 Antibody (G-6): m-IgG Fc BP-HRP Bundle | sc-552037 | 200 µg Ab; 10 µg BP | $354.00 | |||
ARHGAP4 Antibody (G-6) AC | sc-376251 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ARHGAP4 Antibody (G-6) HRP | sc-376251 HRP | 200 µg/ml | $316.00 | |||
ARHGAP4 Antibody (G-6) FITC | sc-376251 FITC | 200 µg/ml | $330.00 | |||
ARHGAP4 Antibody (G-6) PE | sc-376251 PE | 200 µg/ml | $343.00 | |||
ARHGAP4 Antibody (G-6) Alexa Fluor® 488 | sc-376251 AF488 | 200 µg/ml | $357.00 | |||
ARHGAP4 Antibody (G-6) Alexa Fluor® 546 | sc-376251 AF546 | 200 µg/ml | $357.00 | |||
ARHGAP4 Antibody (G-6) Alexa Fluor® 594 | sc-376251 AF594 | 200 µg/ml | $357.00 | |||
ARHGAP4 Antibody (G-6) Alexa Fluor® 647 | sc-376251 AF647 | 200 µg/ml | $357.00 | |||
ARHGAP4 Antibody (G-6) Alexa Fluor® 680 | sc-376251 AF680 | 200 µg/ml | $357.00 | |||
ARHGAP4 Antibody (G-6) Alexa Fluor® 790 | sc-376251 AF790 | 200 µg/ml | $357.00 |