ARFRP1 Antibody (A-8): sc-398568
- ARFRP1 Antibody (A-8) is a mouse monoclonal IgG2b κ ARFRP1 antibody provided at 200 µg/ml
- raised against amino acids 137-201 mapping at the C-terminus of ARFRP1 of human origin
- ARFRP1 Antibody (A-8) is recommended for detection of ARFRP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ARFRP1 Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ARFRP1 Antibody (A-8) for WB applications. These reagents are now offered in bundles with ARFRP1 Antibody (A-8) (see ordering information below).
QUICK LINKS
SEE ALSO...
ARFRP1 Antibody (A-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects ARFRP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARFRP1 (A-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ADP-ribosylation factor (ARF) protein family, to which ARFRP1 belongs, consists of structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins, which are crucial for vesicle trafficking and signal transduction in eukaryotic cells. ARFRP1, also known as ADP-ribosylation factor related protein 1 or ARL18, is a 201 amino acid membrane-associated GTPase that primarily localizes to the plasma membrane and the Golgi apparatus, playing a significant role in maintaining organelle integrity and facilitating the assembly of coat proteins. Notably, ARFRP1 interacts with SYS1, suggesting involvement in plasma membrane-related signaling events. ARFRP1 exists in multiple alternatively spliced isoforms and is encoded by a gene located in a cluster on chromosome 20, which is often overexpressed in tumors, indicating a potential role in carcinogenesis. Understanding ARFRP1 function and interactions is essential for elucidating contributions to cellular processes and implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARFRP1 Antibody (A-8) References:
- The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D. | Schürmann, A., et al. 1999. J Biol Chem. 274: 9744-51. PMID: 10092663
- Overexpression of M68/DcR3 in human gastrointestinal tract tumors independent of gene amplification and its location in a four-gene cluster. | Bai, C., et al. 2000. Proc Natl Acad Sci U S A. 97: 1230-5. PMID: 10655513
- Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p. | Behnia, R., et al. 2004. Nat Cell Biol. 6: 405-13. PMID: 15077113
- Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. | Shin, HW., et al. 2005. J Cell Sci. 118: 4039-48. PMID: 16129887
- Knockout of Arfrp1 leads to disruption of ARF-like1 (ARL1) targeting to the trans-Golgi in mouse embryos and HeLa cells. | Zahn, C., et al. 2006. Mol Membr Biol. 23: 475-85. PMID: 17127620
- ADP-ribosylation factor-like GTPase ARFRP1 is required for trans-Golgi to plasma membrane trafficking of E-cadherin. | Zahn, C., et al. 2008. J Biol Chem. 283: 27179-88. PMID: 18662990
- Distribution of ADP-ribosylation factor-related protein 1 in mouse brain. | Paratore, S., et al. 2008. Arch Ital Biol. 146: 53-61. PMID: 18666447
- ARP is a plasma membrane-associated Ras-related GTPase with remote similarity to the family of ADP-ribosylation factors. | Schürmann, A., et al. 1995. J Biol Chem. 270: 30657-63. PMID: 8530503
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARFRP1 Antibody (A-8) | sc-398568 | 200 µg/ml | $316.00 | |||
ARFRP1 Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-530843 | 200 µg Ab; 10 µg BP | $354.00 | |||
ARFRP1 Antibody (A-8): m-IgGκ BP-HRP Bundle | sc-524391 | 200 µg Ab, 40 µg BP | $354.00 | |||
ARFRP1 Antibody (A-8): m-IgG2b BP-HRP Bundle | sc-550232 | 200 µg Ab; 10 µg BP | $354.00 | |||
ARFRP1 Antibody (A-8) AC | sc-398568 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ARFRP1 Antibody (A-8) HRP | sc-398568 HRP | 200 µg/ml | $316.00 | |||
ARFRP1 Antibody (A-8) FITC | sc-398568 FITC | 200 µg/ml | $330.00 | |||
ARFRP1 Antibody (A-8) PE | sc-398568 PE | 200 µg/ml | $343.00 | |||
ARFRP1 Antibody (A-8) Alexa Fluor® 488 | sc-398568 AF488 | 200 µg/ml | $357.00 | |||
ARFRP1 Antibody (A-8) Alexa Fluor® 546 | sc-398568 AF546 | 200 µg/ml | $357.00 | |||
ARFRP1 Antibody (A-8) Alexa Fluor® 594 | sc-398568 AF594 | 200 µg/ml | $357.00 | |||
ARFRP1 Antibody (A-8) Alexa Fluor® 647 | sc-398568 AF647 | 200 µg/ml | $357.00 | |||
ARFRP1 Antibody (A-8) Alexa Fluor® 680 | sc-398568 AF680 | 200 µg/ml | $357.00 | |||
ARFRP1 Antibody (A-8) Alexa Fluor® 790 | sc-398568 AF790 | 200 µg/ml | $357.00 |