ARFGAP2 Antibody (C-5): sc-376998

ARFGAP2 Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects ARFGAP2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARFGAP2, also known as zinc finger protein 289 or ADP-ribosylation factor GTPase-activating protein 2, plays a crucial role in regulating the activity of ADP-ribosylation factor (ARF) proteins, which are essential for vesicle trafficking and signal transduction in eukaryotic cells. By localizing to the cytoplasmic side of the Golgi apparatus, ARFGAP2 facilitates the hydrolysis of GTP bound to ARF proteins, thereby promoting their inactivation and ensuring proper vesicle formation and transport. This regulation is vital for maintaining organelle integrity and coordinating cellular responses to various stimuli. Furthermore, ARFGAP2′s interaction with COP-I-coated vesicles underscores its importance in the retrograde transport pathway, which is critical for recycling proteins back to the endoplasmic reticulum. ARFGAP2′s ability to modulate ARF activity highlights its significance in cellular homeostasis and the potential implications in various diseases where vesicle trafficking is disrupted.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ARFGAP2 Antibody (C-5) References:

1. Gamma-COP appendage domain - structure and function.  |  Watson, PJ., et al. 2004. Traffic. 5: 79-88. PMID: 14690497
2. Three homologous ArfGAPs participate in coat protein I-mediated transport.  |  Saitoh, A., et al. 2009. J Biol Chem. 284: 13948-13957. PMID: 19299515
3. The GAP domain and the SNARE, coatomer and cargo interaction region of the ArfGAP2/3 Glo3 are sufficient for Glo3 function.  |  Schindler, C., et al. 2009. Traffic. 10: 1362-75. PMID: 19602196
4. Protein networks involved in vesicle fusion, transport, and storage revealed by array-based proteomics.  |  Bauer, M., et al. 2011. Methods Mol Biol. 781: 47-58. PMID: 21877276
5. ArfGAP3 regulates the transport of cation-independent mannose 6-phosphate receptor in the post-Golgi compartment.  |  Shiba, Y., et al. 2013. Curr Biol. 23: 1945-51. PMID: 24076238
6. Activation of Gαi at the Golgi by GIV/Girdin imposes finiteness in Arf1 signaling.  |  Lo, IC., et al. 2015. Dev Cell. 33: 189-203. PMID: 25865347
7. The Arf-GDP-regulated recruitment of GBF1 to Golgi membranes requires domains HDS1 and HDS2 and a Golgi-localized protein receptor.  |  Quilty, D., et al. 2018. J Cell Sci. 132: PMID: 29507113
8. The Glo3 GAP crystal structure supports the molecular niche model for ArfGAPs in COPI coats.  |  Xie, B., et al. 2021. Adv Biol Regul. 79: 100781. PMID: 33436318
9. MIF Variant rs755622 Is Associated with Severe Crohn's Disease and Better Response to Anti-TNF Adalimumab Therapy.  |  Jezernik, G., et al. 2023. Genes (Basel). 14: PMID: 36833379
10. Label-free quantitative proteomics reveals the mechanisms of Aurora kinase B in renal cell carcinoma.  |  Li, Y. and Yang, Y. 2024. SAGE Open Med. 12: 20503121241228474. PMID: 38516642

The preferred ARFGAP2 antibody is ARFGAP2 Antibody (F-11): sc-377049.