ARFGAP2 Antibody (F-11): sc-377049
- ARFGAP2 Antibody (F-11) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 311-430 mapping near the C-terminus of ARFGAP2 of human origin
- Anti-ARFGAP2 Antibody (F-11) is recommended for detection of ARFGAP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ARFGAP2 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ARFGAP2 Antibody (F-11) for WB and IHC(P) applications. These reagents are now offered in bundles with ARFGAP2 Antibody (F-11) (see ordering information below).
QUICK LINKS
ARFGAP2 Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects ARFGAP2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ARFGAP2 (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ADP-ribosylation factor (ARF) protein family, to which ARFGAP2 belongs, plays a crucial role in vesicle trafficking and signal transduction within eukaryotic cells, making ARFGAP2 essential for maintaining cellular homeostasis. Specifically, ARFGAP2 functions as a GTPase-activating protein (GAP) for ARF family proteins, facilitating the hydrolysis of GTP to GDP, which is vital for the regulation of ARF activity and, consequently, vesicle formation and transport. This regulatory mechanism is particularly important as ARFGAP2 influences the coordination of spectrin interactions with Golgi membranes and the recruitment of actin to the Golgi apparatus, thereby impacting the integrity and functionality of cellular organelles. Localized to the cytoplasmic side of the Golgi apparatus, ARFGAP2 contains one ARF-GAP domain and is associated with COP-I-coated vesicles, underscoring ARFGAP2′s significance in intracellular trafficking processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARFGAP2 Antibody (F-11) References:
- Activated ADP-ribosylation factor assembles distinct pools of actin on golgi membranes. | Fucini, RV., et al. 2000. J Biol Chem. 275: 18824-9. PMID: 10777475
- Molecular cloning and characterization of a zinc finger protein involved in Id-1-stimulated mammary epithelial cell growth. | Singh, J., et al. 2001. J Biol Chem. 276: 11852-8. PMID: 11278321
- Gamma-COP appendage domain - structure and function. | Watson, PJ., et al. 2004. Traffic. 5: 79-88. PMID: 14690497
- Two human ARFGAPs associated with COP-I-coated vesicles. | Frigerio, G., et al. 2007. Traffic. 8: 1644-55. PMID: 17760859
- Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking. | Weimer, C., et al. 2008. J Cell Biol. 183: 725-35. PMID: 19015319
- Discrete determinants in ArfGAP2/3 conferring Golgi localization and regulation by the COPI coat. | Kliouchnikov, L., et al. 2009. Mol Biol Cell. 20: 859-69. PMID: 19109418
- ARFGAP2 and ARFGAP3 are essential for COPI coat assembly on the Golgi membrane of living cells. | Kartberg, F., et al. 2010. J Biol Chem. 285: 36709-20. PMID: 20858901
- Distinct role of subcomplexes of the COPI coat in the regulation of ArfGAP2 activity. | Pevzner, I., et al. 2012. Traffic. 13: 849-56. PMID: 22375848
- The ArfGAP2/3 Glo3 and ergosterol collaborate in transport of a subset of cargoes. | Estrada, AF., et al. 2015. Biol Open. 4: 792-802. PMID: 25964658
- The amino terminus of ADP-ribosylation factor (ARF) 1 is essential for interaction with Gs and ARF GTPase-activating protein. | Randazzo, PA., et al. 1994. J Biol Chem. 269: 29490-4. PMID: 7961931
- Structure of the human ADP-ribosylation factor 1 complexed with GDP. | Amor, JC., et al. 1994. Nature. 372: 704-8. PMID: 7990966
- ADP ribosylation factor regulates spectrin binding to the Golgi complex. | Godi, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 8607-12. PMID: 9671725
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARFGAP2 Antibody (F-11) | sc-377049 | 200 µg/ml | $316.00 | |||
ARFGAP2 Antibody (F-11): m-IgGκ BP-HRP Bundle | sc-523169 | 200 µg Ab, 40 µg BP | $354.00 | |||
ARFGAP2 Antibody (F-11): m-IgG2a BP-HRP Bundle | sc-546148 | 200 µg Ab; 10 µg BP | $354.00 | |||
ARFGAP2 Antibody (F-11) AC | sc-377049 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ARFGAP2 Antibody (F-11) HRP | sc-377049 HRP | 200 µg/ml | $316.00 | |||
ARFGAP2 Antibody (F-11) FITC | sc-377049 FITC | 200 µg/ml | $330.00 | |||
ARFGAP2 Antibody (F-11) PE | sc-377049 PE | 200 µg/ml | $343.00 | |||
ARFGAP2 Antibody (F-11) Alexa Fluor® 488 | sc-377049 AF488 | 200 µg/ml | $357.00 | |||
ARFGAP2 Antibody (F-11) Alexa Fluor® 546 | sc-377049 AF546 | 200 µg/ml | $357.00 | |||
ARFGAP2 Antibody (F-11) Alexa Fluor® 594 | sc-377049 AF594 | 200 µg/ml | $357.00 | |||
ARFGAP2 Antibody (F-11) Alexa Fluor® 647 | sc-377049 AF647 | 200 µg/ml | $357.00 | |||
ARFGAP2 Antibody (F-11) Alexa Fluor® 680 | sc-377049 AF680 | 200 µg/ml | $357.00 | |||
ARFGAP2 Antibody (F-11) Alexa Fluor® 790 | sc-377049 AF790 | 200 µg/ml | $357.00 |