ARC Antibody (G-4): sc-398121

ARC Antibody (G-4) is a mouse monoclonal IgG2b antibody that detects ARC in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARC, also known as apoptosis repressor with CARD domain or nucleolar protein 3 (NOL3, NOP, NOP30), plays a crucial role in regulating apoptosis by inhibiting caspases, specifically caspase-2 and caspase-8, which are key mediators of programmed cell death. ARC′s ability to prevent apoptosis is vital for maintaining cellular homeostasis and survival, particularly in tissues like skeletal muscle and heart, where ARC expression is critical for cell viability under stress conditions. Notably, ARC is phosphorylated at Threonine 149 by casein kinase 2 (CK2), a modification that enhances anti-apoptotic function and facilitates mitochondrial localization, where ARC can effectively interact with caspase-8. This localization allows ARC to directly inhibit apoptosis triggered by various death receptors, including CD95/FAS and tumor necrosis factor receptor-1, thereby playing a protective role in cellular responses to stress and injury. With multifaceted functions and critical interactions, ARC is an important protein in the study of apoptosis and cellular survival mechanisms, making anti-ARC antibody (G-4) an essential tool for researchers investigating these pathways.

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ARC Antibody (G-4) References:

1. Calcium binding of ARC mediates regulation of caspase 8 and cell death.  |  Jo, DG., et al. 2004. Mol Cell Biol. 24: 9763-70. PMID: 15509781
2. p53 initiates apoptosis by transcriptionally targeting the antiapoptotic protein ARC.  |  Li, YZ., et al. 2008. Mol Cell Biol. 28: 564-74. PMID: 17998337
3. Regulation of p53 tetramerization and nuclear export by ARC.  |  Foo, RS., et al. 2007. Proc Natl Acad Sci U S A. 104: 20826-31. PMID: 18087040
4. Inhibition of endoplasmic reticulum stress-induced apoptosis of melanoma cells by the ARC protein.  |  Chen, LH., et al. 2008. Cancer Res. 68: 834-42. PMID: 18245485
5. Mitochondrial fission leads to Smac/DIABLO release quenched by ARC.  |  Li, J., et al. 2010. Apoptosis. 15: 1187-96. PMID: 20552279
6. ARC is a novel therapeutic approach against acetaminophen-induced hepatocellular necrosis.  |  An, J., et al. 2013. J Hepatol. 58: 297-305. PMID: 23046676
7. A novel role for the apoptosis inhibitor ARC in suppressing TNFα-induced regulated necrosis.  |  Kung, G., et al. 2014. Cell Death Differ. 21: 634-44. PMID: 24440909
8. Functional, morphological, and apoptotic alterations in skeletal muscle of ARC deficient mice.  |  Mitchell, AS., et al. 2015. Apoptosis. 20: 310-26. PMID: 25596718
9. Anti-apoptotic ARC protein confers chemoresistance by controlling leukemia-microenvironment interactions through a NFκB/IL1β signaling network.  |  Carter, BZ., et al. 2016. Oncotarget. 7: 20054-67. PMID: 26956049
10. The effect of ARC ablation on skeletal muscle morphology, function, and apoptotic signaling during aging.  |  Vorobej, K., et al. 2018. Exp Gerontol. 101: 69-79. PMID: 29056555

The preferred ARC antibody is ARC Antibody (F-11): sc-374177.