Aquaporin 5/AQP5 Antibody (D-7): sc-514022
- Aquaporin 5/AQP5 Antibody (D-7) is a mouse monoclonal IgG1 κ Aquaporin 5/AQP5 antibody, cited in 38 publications, provided at 200 µg/ml
- raised against amino acids 66-265 mapping at the C-terminus of AQP5 of human origin
- Aquaporin 5/AQP5 Antibody (D-7) is recommended for detection of AQP5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Aquaporin 5/AQP5 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Aquaporin 5/AQP5 Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Aquaporin 5/AQP5 Antibody (D-7) (see ordering information below).
AQP5 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects AQP5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-AQP5 antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AQP5, a member of the aquaporin family, plays a crucial role in facilitating water transport across cell membranes, particularly in the lungs, where AQP5 is predominantly expressed in type I alveolar epithelial cells. This function is vital for maintaining proper hydration and surfactant homeostasis in the alveoli, which is essential for efficient gas exchange and overall respiratory function. Dysregulation of AQP5 has been implicated in various pulmonary disorders, highlighting AQP5′s importance in both normal physiology and disease states. AQP5′s ability to transport water rapidly makes AQP5 a key player in processes such as pulmonary secretion and the regulation of airway surface liquid, further underscoring AQP5′s significance in respiratory health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Aquaporin 5/AQP5 Antibody (D-7) References:
- The mammalian aquaporin water channel family: A promising new drug target. | Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
- Lung fluid transport in aquaporin-5 knockout mice. | Ma, T., et al. 2000. J Clin Invest. 105: 93-100. PMID: 10619865
- Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. | Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
- AQP5 enriches for stem cells and cancer origins in the distal stomach. | Tan, SH., et al. 2020. Nature. 578: 437-443. PMID: 32025032
- AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitination. | Zhao, R., et al. 2022. J Exp Clin Cancer Res. 41: 322. PMID: 36372898
- NNMT enriches for AQP5+ cancer stem cells to drive malignant progression in early gastric cardia adenocarcinoma. | Wang, Z., et al. 2023. Gut. 73: 63-77. PMID: 36977555
- AQP5 deficiency promotes the senescence of lens epithelial cells through mitochondrial dysfunction. | Zhang, K., et al. 2023. Biochem Biophys Res Commun. 680: 184-193. PMID: 37742347
- Structure and chromosomal localization of a human water channel (AQP3) gene. | Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
- cDNA cloning, gene organization, and chromosomal localization of a human mercurial insensitive water channel. Evidence for distinct transcriptional units. | Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
- Requirement of human renal water channel aquaporin-2 for vasopressin-dependent concentration of urine. | Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
- The human Aquaporin-5 gene. Molecular characterization and chromosomal localization. | Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
- Aquaporins. | Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 5/AQP5 Antibody (D-7) | sc-514022 | 200 µg/ml | $322.00 | |||
Aquaporin 5/AQP5 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-531004 | 200 µg Ab; 10 µg BP | $361.00 | |||
Aquaporin 5/AQP5 Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-524575 | 200 µg Ab, 40 µg BP | $361.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) AC | sc-514022 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) HRP | sc-514022 HRP | 200 µg/ml | $322.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) FITC | sc-514022 FITC | 200 µg/ml | $336.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) PE | sc-514022 PE | 200 µg/ml | $349.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) Alexa Fluor® 488 | sc-514022 AF488 | 200 µg/ml | $364.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) Alexa Fluor® 546 | sc-514022 AF546 | 200 µg/ml | $364.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) Alexa Fluor® 594 | sc-514022 AF594 | 200 µg/ml | $364.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) Alexa Fluor® 647 | sc-514022 AF647 | 200 µg/ml | $364.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) Alexa Fluor® 680 | sc-514022 AF680 | 200 µg/ml | $364.00 | |||
Aquaporin 5/AQP5 Antibody (D-7) Alexa Fluor® 790 | sc-514022 AF790 | 200 µg/ml | $364.00 |