Aquaporin 3/AQP3 Antibody (F-1): sc-518001

AQP3 (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects AQP3 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). AQP3 (F-1) antibody is available as both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate water transport through cell membranes, with this function conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10, with multiple types commonly present in the same cell. While most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is uniquely activated by vasopressin to enhance water reabsorption in kidney collecting ducts. Aquaporins play crucial roles in renal water absorption, pulmonary secretion generation, lacrimation, and cerebrospinal fluid and aqueous humor secretion and reabsorption. AQP3 is expressed in collecting duct cells′ basolateral membrane in the kidney.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Aquaporin 3/AQP3 Antibody (F-1) References:

1. The mammalian aquaporin water channel family: A promising new drug target.  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
2. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family.  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
3. Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells.  |  Ishibashi, K., et al. 1994. Proc Natl Acad Sci U S A. 91: 6269-73. PMID: 7517548
4. Structure and chromosomal localization of a human water channel (AQP3) gene.  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
5. cDNA cloning, gene organization, and chromosomal localization of a human mercurial insensitive water channel. Evidence for distinct transcriptional units.  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
6. Requirement of human renal water channel aquaporin-2 for vasopressin-dependent concentration of urine.  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
7. The human Aquaporin-5 gene. Molecular characterization and chromosomal localization.  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
8. Localization of the human gene for aquaporin 3 (AQP3) to chromosome 9, region p21-->p12, using fluorescent in situ hybridization.  |  Mulders, SM., et al. 1996. Cytogenet Cell Genet. 72: 303-5. PMID: 8641135
9. Aquaporins.  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aquaporin 3/AQP3 Antibody (F-1) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-Aquaporin 3/AQP3 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aquaporin 3/AQP3 Antibody (F-1) Specifications

Alternate Product Name: aquaporin 3

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 268-292

Epitope Target: AQP3

Epitope Species: human

Product Reactivity/Detection: AQP3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for Aquaporin 3/AQP3 Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin