APPL2 Antibody (B-12): sc-398860

APPL2 Antibody (B-12) is a mouse monoclonal IgG2b antibody that detects APPL2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APPL2, also known as adaptor protein containing pH domain, PTB domain, and leucine zipper motif 2, plays a crucial role in cellular signaling by linking epidermal growth factor (EGF) receptor activation to downstream chromatin remodeling processes in the nucleus. APPL2 is essential for cell growth and proliferation, as anti-APPL2 antibody (B-12) reveals interactions with the NuRD/MeCP1 complex, facilitating the regulation of gene expression. Additionally, APPL2 associates with the follicle-stimulating hormone receptor, highlighting involvement in reproductive signaling pathways. APPL2 is predominantly expressed in vital organs such as the heart, brain, skeletal muscle, and kidney, underscoring importance in various physiological functions. With a 54% homology to APPL1, APPL2′s structural similarities suggest a conserved mechanism of action within the APPL family, making APPL2 monoclonal antibody (B-12) a valuable tool for researchers studying EGF signaling and cellular processes.

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APPL2 Antibody (B-12) References:

1. APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex.  |  Nechamen, CA., et al. 2007. Mol Cell Endocrinol. 260-262: 93-9. PMID: 17030088
2. Regulation of B-lymphocyte activation by the PH domain adaptor protein Bam32/DAPP1.  |  Marshall, AJ., et al. 2007. Biochem Soc Trans. 35: 181-2. PMID: 17371232
3. Endosomal adaptor proteins APPL1 and APPL2 are novel activators of beta-catenin/TCF-mediated transcription.  |  Rashid, S., et al. 2009. J Biol Chem. 284: 18115-28. PMID: 19433865
4. Absence of Appl2 sensitizes endotoxin shock through activation of PI3K/Akt pathway.  |  Mao, L., et al. 2014. Cell Biosci. 4: 60. PMID: 25328665
5. Appl1 and Appl2 are Expendable for Mouse Development But Are Essential for HGF-Induced Akt Activation and Migration in Mouse Embryonic Fibroblasts.  |  Tan, Y., et al. 2016. J Cell Physiol. 231: 1142-50. PMID: 26445298
6. Baicalin Modulates APPL2/Glucocorticoid Receptor Signaling Cascade, Promotes Neurogenesis, and Attenuates Emotional and Olfactory Dysfunctions in Chronic Corticosterone-Induced Depression.  |  Gao, C., et al. 2018. Mol Neurobiol. 55: 9334-9348. PMID: 29675572
7. The reversal effect of physical exercise on aging-related increases in APPL2 content in skeletal muscle.  |  Canciglieri, PH., et al. 2018. Life Sci. 210: 209-213. PMID: 30189216
8. Overexpressed in colorectal carcinoma gene (OCC-1) upregulation and APPL2 gene downregulation in breast cancer specimens.  |  Ghalaei, A., et al. 2018. Mol Biol Rep. 45: 1889-1895. PMID: 30218350
9. Crucial Role of the SH2B1 PH Domain for the Control of Energy Balance.  |  Flores, A., et al. 2019. Diabetes. 68: 2049-2062. PMID: 31439647
10. APPL2 Negatively Regulates Olfactory Functions by Switching Fate Commitments of Neural Stem Cells in Adult Olfactory Bulb via Interaction with Notch1 Signaling.  |  Gao, C., et al. 2020. Neurosci Bull. 36: 997-1008. PMID: 32468397

The preferred APPL2 antibody is APPL2 Antibody (F-2): sc-398859.