API5 Antibody (E-12): sc-374528

API5 Antibody (E-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects API5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). API5 (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. API5, also known as apoptosis inhibitor 5, AAC11, FIF, MIG8, XAGL, or API5L1, is a crucial antiapoptotic nuclear protein that plays a significant role in cellular survival and proliferation, making API5 a key player in cancer biology. API5 structure includes a nuclear localization sequence, an LxxLL motif, a leucine zipper domain, and a transactivation domain flanked by two acidic domains, which are essential for API5 function in regulating gene expression. Nuclear localization of API5 allows interaction with various transcription factors and signaling molecules, including forming a complex with fibroblast growth factor 2 (FGF-2), thereby mediating FGF-2-dependent signaling pathways. This interaction is vital for the regulation of matrix metalloproteinase-2 synthesis and the suppression of E2F-dependent apoptosis, linking API5 to tumor progression and malignancy in various cancer cell lines. API5 ability to modulate apoptotic pathways underscores API5 importance in cancer research and therapeutic development, making anti-API5 antibody (E-12) an invaluable tool for studying API5 role in cellular processes and disease mechanisms.

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API5 Antibody (E-12) References:

1. Molecular cloning and fine mapping of API5L1, a novel human gene strongly related to an antiapoptotic gene.  |  Gianfrancesco, F., et al. 1999. Cytogenet Cell Genet. 84: 164-6. PMID: 10393420
2. AAC-11 overexpression induces invasion and protects cervical cancer cells from apoptosis.  |  Kim, JW., et al. 2000. Lab Invest. 80: 587-94. PMID: 10780674
3. FIF [fibroblast growth factor-2 (FGF-2)-interacting-factor], a nuclear putatively antiapoptotic factor, interacts specifically with FGF-2.  |  Van den Berghe, L., et al. 2000. Mol Endocrinol. 14: 1709-24. PMID: 11075807
4. Expression of the antiapoptosis gene, AAC-11, as a prognosis marker in non-small cell lung cancer.  |  Sasaki, H., et al. 2001. Lung Cancer. 34: 53-7. PMID: 11557113
5. Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo.  |  Morris, EJ., et al. 2006. PLoS Genet. 2: e196. PMID: 17112319
6. The antiapoptotic protein Api5 and its partner, high molecular weight FGF2, are up-regulated in B cell chronic lymphoid leukemia.  |  Krejci, P., et al. 2007. J Leukoc Biol. 82: 1363-4. PMID: 17827341
7. AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal.  |  Tewari, M., et al. 1997. Cancer Res. 57: 4063-9. PMID: 9307294
8. Identification of genes differentially expressed in vascular smooth muscle cells following benzo[a]pyrene challenge: implications for chemical atherogenesis.  |  Lu, KP. and Ramos, KS. 1998. Biochem Biophys Res Commun. 253: 828-33. PMID: 9918813

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-API5 Antibody (E-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-API5 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

API5 Antibody (E-12) Specifications

Alternate Product Name: API5

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: API5

Epitope Species: human

Product Reactivity/Detection: API5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for API5 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin