Apg-2 Antibody (F-10): sc-365140

Apg-2 Antibody (F-10) is a mouse monoclonal IgG1 antibody that detects Apg-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Apg-2, a member of the heat shock protein 110 family, plays a crucial role in cellular stress responses by acting as a molecular chaperone, facilitating proper folding and assembly of proteins, and preventing aggregation under stress conditions. This function helps maintain cellular homeostasis and protects cells from damage caused by misfolded proteins, which can lead to various diseases, including neurodegenerative disorders. Apg-2 is predominantly located in the cytoplasm and can translocate to the nucleus under stress, highlighting a dynamic role in cellular function and response to environmental changes. Apg-2 interactions with other proteins, such as HSP 70 and HSP 90, underscore significance in the chaperone network, collaborating to ensure protein quality control and cellular resilience. Anti-Apg-2 antibody (F-10) serves as an essential tool for researchers studying protein folding mechanisms and cellular response to stress, providing insights into the pathophysiology of diseases associated with protein misfolding.

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Apg-2 Antibody (F-10) References:

1. Cloning of human cDNAs for Apg-1 and Apg-2, members of the Hsp110 family, and chromosomal assignment of their genes.  |  Nonoguchi, K., et al. 1999. Gene. 237: 21-8. PMID: 10524232
2. Expression of APG-2 protein, a member of the heat shock protein 110 family, in developing rat brain.  |  Okui, M., et al. 2000. Neurochem Int. 36: 35-43. PMID: 10566957
3. Apg2 is a novel protein required for the cytoplasm to vacuole targeting, autophagy, and pexophagy pathways.  |  Wang, CW., et al. 2001. J Biol Chem. 276: 30442-51. PMID: 11382760
4. Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas.  |  Gotoh, K., et al. 2004. FEBS Lett. 560: 19-24. PMID: 14987991
5. The heat-shock protein Apg-2 binds to the tight junction protein ZO-1 and regulates transcriptional activity of ZONAB.  |  Tsapara, A., et al. 2006. Mol Biol Cell. 17: 1322-30. PMID: 16407410
6. Overexpression of Apg-2 increases cell proliferation and protects from oxidative damage in BaF3-BCR/ABL cells.  |  Li, C., et al. 2010. Int J Oncol. 36: 899-904. PMID: 20198334
7. Proteomic-based identification of Apg-2 as a therapeutic target for chronic myeloid leukemia.  |  Li, Y., et al. 2013. Cell Signal. 25: 2604-12. PMID: 24012954
8. Calibration and characterization of intracellular Asante Potassium Green probes, APG-2 and APG-4.  |  Rana, PS., et al. 2019. Anal Biochem. 567: 8-13. PMID: 30503709
9. Regulation of Human Hsc70 ATPase and Chaperone Activities by Apg2: Role of the Acidic Subdomain.  |  Cabrera, Y., et al. 2019. J Mol Biol. 431: 444-461. PMID: 30521813
10. Fine-tuning of the Hsc70-based Human Protein Disaggregase Machinery by the Distinctive C-terminal Extension of Apg2.  |  Cabrera, Y., et al. 2022. J Mol Biol. 434: 167841. PMID: 36167183

The preferred HSP 105 antibody is HSP 105 Antibody (B-7): sc-74550.