Apg-1 Antibody (D-12): sc-133253

Apg-1 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Apg-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Apg-1 (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Apg-1, also known as HSPA4L (heat shock 70 kDa protein 4-like) or Osp94 (osmotic stress protein 94), is a member of the heat shock protein (HSP) family, which plays a crucial role in cellular stress responses. Specifically, Apg-1 regulates protein folding and prevents protein aggregation, which is vital for maintaining cellular homeostasis, especially under stress conditions such as heat shock. Apg-1 forms an 839 amino acid homodimer that primarily localizes to the cytoplasm and nucleus, with the potential to translocate to the nucleus in response to stress, highlighting its importance in cellular signaling and stress response mechanisms. Apg-1′s ability to interact with other proteins and assist in their proper folding underscores its significance in various biological processes, making Apg-1 a valuable target for research in cellular biology and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Apg-1 Antibody (D-12) References:

1. Germ cell-specific heat shock protein 105 binds to p53 in a temperature-sensitive manner in rat testis.  |  Kumagai, J., et al. 2000. Eur J Biochem. 267: 3073-8. PMID: 10806408
2. Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy.  |  Abeliovich, H., et al. 2003. Mol Biol Cell. 14: 477-90. PMID: 12589048
3. Effects of low culture temperature on the induction of hsp70 mRNA and the accumulation of hsp70 and hsp105 in mouse FM3A cells.  |  Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
4. Hspa4l-deficient mice display increased incidence of male infertility and hydronephrosis development.  |  Held, T., et al. 2006. Mol Cell Biol. 26: 8099-108. PMID: 16923965
5. Respiratory distress and early neonatal lethality in Hspa4l/Hspa4 double-mutant mice.  |  Mohamed, BA., et al. 2014. Am J Respir Cell Mol Biol. 50: 817-24. PMID: 23980576
6. Role of Apg-1 in HSF1 activation and bortezomib sensitivity in myeloma cells.  |  Zhu, M., et al. 2020. Exp Hematol. 81: 50-59. PMID: 31899217
7. Novel function of the C-terminal region of the Hsp110 family member Osp94 in unfolded protein refolding.  |  Kojima, R., et al. 2022. J Cell Sci. 135: PMID: 35237814
8. Dynamics of the chaperonin ATPase cycle: implications for facilitated protein folding.  |  Todd, MJ., et al. 1994. Science. 265: 659-66. PMID: 7913555
9. Role of the major heat shock proteins as molecular chaperones.  |  Georgopoulos, C. and Welch, WJ. 1993. Annu Rev Cell Biol. 9: 601-34. PMID: 8280473
10. Cloning and expression of murine high molecular mass heat shock proteins, HSP105.  |  Yasuda, K., et al. 1995. J Biol Chem. 270: 29718-23. PMID: 8530361
11. Cloning of apg-2 encoding a novel member of heat shock protein 110 family.  |  Kaneko, Y., et al. 1997. Gene. 189: 19-24. PMID: 9161406
12. Induction of Apg-1, a member of the heat shock protein 110 family, following transient forebrain ischemia in the rat brain.  |  Xue, JH., et al. 1998. Biochem Biophys Res Commun. 247: 796-801. PMID: 9647773

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Apg-1 Antibody (D-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Apg-1 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Apg-1 Antibody (D-12) Specifications

Alternate Product Name: Apg-1

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 231-838

Epitope Target: Apg-1

Epitope Species: human

Product Reactivity/Detection: Apg-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Apg-1 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Apg-2 antibody is Apg-2 Antibody (A-7): sc-365366.