Apg-2 Antibody (A-7) recommended for detection of Apg-2 of mouse, rat and human origin by WB, IP, IF and ELISA

Apg-2 Antibody (A-7): sc-365366

Apg-2 Antibody (A-7) is rated 3.8 out of 5 by 5.
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Datasheets

    • Anti-Apg-2 Antibody (A-7) is a mouse monoclonal IgG1 (kappa light chain) Apg-2 antibody provided at 200 µg/ml
    • raised against amino acids 60-551 of Apg-2 of mouse origin
    • Anti-Apg-2 Antibody (A-7) is recommended for detection of Apg-2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Apg-2 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Apg-2 (A-7): sc-365366.

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-365366: Apg-2 (A-7) mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from It did not work for human APG-2 I tested it for detecting human APG-2 after siRNA treatment and after APG-2 overexpression. I didn't get any signal on WB.
Date published: 2017-03-29
Rated 3 out of 5 by from IF in Mouse testis Immunofluorescence staining of paraffin-embedded mouse testis showing High background
Date published: 2017-02-21
Rated 5 out of 5 by from Excellent antibody for Western blotting. This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detects an appropriately sized target protein in immunoblots.
Date published: 2016-10-13
Rated 5 out of 5 by from Very strong and specific band detected at Very strong and specific band detected at expected molecular weight in F9 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-02-18
Rated 5 out of 5 by from WB publishable data with hamster CHO WB publishable data with hamster CHO-K1 cells - SCBT Publication Review
Date published: 2014-08-28
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