APEH Antibody (G-3): sc-376577

APEH Antibody (G-3) is a mouse monoclonal IgG1 antibody that detects APEH (Acyl-peptide hydrolase) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APEH is a 732 amino acid cytoplasmic protein that functions as a homotetramer, playing a crucial role in the hydrolysis of N-terminal acetylated amino acids from small acetylated peptides. This enzymatic activity facilitates the subsequent processing of the acetyl amino acid by aminoacylases, leading to the production of acetate and free amino acids, which are vital for various metabolic processes. The gene encoding human APEH is located on chromosome 3, a region often deleted in several cancers, including renal cell carcinoma and small cell lung carcinoma, indicating a potential role for APEH in tumorigenesis. Chromosome 3 encompasses approximately 214 million bases and houses over 1,100 genes, including critical tumor suppressor genes such as RASSF1, which mediates apoptosis, HYAL1, which regulates cell migration, and SEMA3B, known for its role in suppressing angiogenesis. Anti-APEH antibody (G-3) is an essential tool for researchers investigating the implications of APEH in cancer biology and metabolic regulation.

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APEH Antibody (G-3) References:

1. RedOx status, proteasome and APEH: insights into anticancer mechanisms of t10,c12-conjugated linoleic acid isomer on A375 melanoma cells.  |  Bergamo, P., et al. 2013. PLoS One. 8: e80900. PMID: 24260504
2. APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome.  |  Palumbo, R., et al. 2016. Int J Mol Sci. 17: PMID: 27669226
3. Identification of Lung Inflammation-Related Elevation of Acylamino Acid Releasing Enzyme (APEH) Activity Using an Enzymomics Approach.  |  Komatsu, T., et al. 2016. Chem Pharm Bull (Tokyo). 64: 1533-1538. PMID: 27803464
4. Acylpeptide hydrolase is a component of the cellular response to DNA damage.  |  Zeng, Z., et al. 2017. DNA Repair (Amst). 58: 52-61. PMID: 28866241
5. Acylpeptide hydrolase is a novel regulator of KRAS plasma membrane localization and function.  |  Tan, L., et al. 2019. J Cell Sci. 132: PMID: 31266814
6. Acylpeptide hydrolase (APEH) sequence variants with potential impact on the metabolism of the antiepileptic drug valproic acid.  |  Tsortouktzidis, D., et al. 2019. Metab Brain Dis. 34: 1629-1634. PMID: 31363986
7. Deubiquitinase UCHL1 Maintains Protein Homeostasis through the PSMA7-APEH-Proteasome Axis in High-grade Serous Ovarian Carcinoma.  |  Tangri, A., et al. 2021. Mol Cancer Res. 19: 1168-1181. PMID: 33753553
8. Acyl Peptide Enzyme Hydrolase (APEH) activity is inhibited by lipid metabolites and peroxidation products.  |  Tyler, K., et al. 2021. Chem Biol Interact. 348: 109639. PMID: 34508712
9. Oxidized Substrates of APEH as a Tool to Study the Endoprotease Activity of the Enzyme.  |  Sandomenico, A., et al. 2021. Int J Mol Sci. 23: PMID: 35008880
10. Prodrug activation in malaria parasites mediated by an imported erythrocyte esterase, acylpeptide hydrolase (APEH).  |  Sundararaman, SA., et al. 2024. bioRxiv.. PMID: 39257815

The preferred APEH antibody is APEH Antibody (G-6): sc-376612.