APC7 Antibody (A-6): sc-365649
- APC7 Antibody (A-6) is a mouse monoclonal IgG1 κ APC7 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 266-565 mapping at the C-terminus of APC7 of human origin
- APC7 Antibody (A-6) is recommended for detection of APC7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-APC7 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for APC7 Antibody (A-6) for WB and IHC(P) applications. These reagents are now offered in bundles with APC7 Antibody (A-6) (see ordering information below).
APC7 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects APC7 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). APC7 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The anaphase-promoting complex (APC), which includes more than ten subunits such as APC1, APC2, APC4, APC5, APC7, APC8, APC10, and APC11, plays a crucial role in cell cycle regulation by facilitating the separation of sister chromatids during the transition from metaphase to anaphase in mitosis. APC7, also known as ANAPC7, mediates interaction between APC and transcription coactivators CBP and p300, which are essential for gene expression regulation during cell division. Proper functioning of APC7 and the entire APC complex is vital for maintaining genomic stability, as dysregulation can lead to aneuploidy and contribute to tumorigenesis. APC is activated through phosphorylation by protein kinases such as Cdk1/cyclin B and polo-like kinase (Plk), with activity tightly regulated by factors like CDC20, CDH1, and MAD2, which ensure accurate cell cycle progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
APC7 Antibody (A-6) References:
- Subunits and substrates of the anaphase-promoting complex. | Peters, JM. 1999. Exp Cell Res. 248: 339-49. PMID: 10222126
- Control of mitotic transitions by the anaphase-promoting complex. | Fang, G., et al. 1999. Philos Trans R Soc Lond B Biol Sci. 354: 1583-90. PMID: 10582244
- The anaphase-promoting complex: new subunits and regulators. | Page, AM. and Hieter, P. 1999. Annu Rev Biochem. 68: 583-609. PMID: 10872461
- Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. | Jörgensen, PM., et al. 2001. Gene. 262: 51-9. PMID: 11179667
- The cyclin-ubiquitin ligase activity of cyclosome/APC is jointly activated by protein kinases Cdk1-cyclin B and Plk. | Golan, A., et al. 2002. J Biol Chem. 277: 15552-7. PMID: 11859075
- Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2. | Bolte, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 4385-90. PMID: 11917129
- Cross-talk between APC/C and CBP/p300. | Liu, J. and Fuchs, SY. 2006. Cancer Biol Ther. 5: 760-2. PMID: 16861917
- The expression pattern of APC2 and APC7 in various cancer cell lines and AML patients. | Rahimi, H., et al. 2015. Adv Med Sci. 60: 259-63. PMID: 26046517
- Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint. | Wild, T., et al. 2018. Cell Rep. 25: 2317-2328.e5. PMID: 30485802
- APC7 mediates ubiquitin signaling in constitutive heterochromatin in the developing mammalian brain. | Ferguson, CJ., et al. 2022. Mol Cell. 82: 90-105.e13. PMID: 34942119
- The Secret Life of the Inhibitor of Virus Replication. | Palukaitis, P., et al. 2022. Viruses. 14: PMID: 36560786
- A subunit of the anaphase-promoting complex is a centromere-associated protein in mammalian cells. | Jörgensen, PM., et al. 1998. Mol Cell Biol. 18: 468-76. PMID: 9418894
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APC7 Antibody (A-6) | sc-365649 | 200 µg/ml | $322.00 | |||
APC7 Antibody (A-6): m-IgG Fc BP-HRP Bundle | sc-529424 | 200 µg Ab; 10 µg BP | $361.00 | |||
APC7 Antibody (A-6): m-IgGκ BP-HRP Bundle | sc-522311 | 200 µg Ab, 40 µg BP | $361.00 | |||
APC7 Antibody (A-6): m-IgG1 BP-HRP Bundle | sc-543509 | 200 µg Ab; 20 µg BP | $361.00 | |||
APC7 Antibody (A-6) AC | sc-365649 AC | 500 µg/ml, 25% agarose | $424.00 | |||
APC7 Antibody (A-6) HRP | sc-365649 HRP | 200 µg/ml | $322.00 | |||
APC7 Antibody (A-6) FITC | sc-365649 FITC | 200 µg/ml | $336.00 | |||
APC7 Antibody (A-6) PE | sc-365649 PE | 200 µg/ml | $349.00 | |||
APC7 Antibody (A-6) Alexa Fluor® 488 | sc-365649 AF488 | 200 µg/ml | $364.00 | |||
APC7 Antibody (A-6) Alexa Fluor® 546 | sc-365649 AF546 | 200 µg/ml | $364.00 | |||
APC7 Antibody (A-6) Alexa Fluor® 594 | sc-365649 AF594 | 200 µg/ml | $364.00 | |||
APC7 Antibody (A-6) Alexa Fluor® 647 | sc-365649 AF647 | 200 µg/ml | $364.00 | |||
APC7 Antibody (A-6) Alexa Fluor® 680 | sc-365649 AF680 | 200 µg/ml | $364.00 | |||
APC7 Antibody (A-6) Alexa Fluor® 790 | sc-365649 AF790 | 200 µg/ml | $364.00 |