APC Antibody (ALi 12-28): sc-53165

APC Antibody (ALi 12-28) is a mouse monoclonal IgG1 kappa light chain antibody that detects APC protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-APC antibody (ALi 12-28) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The adenomatous polyposis coli (APC) protein plays a crucial role in regulating cell adhesion and maintaining the integrity of epithelial tissues, making APC vital for preventing tumorigenesis. Mutations in the APC gene are commonly associated with familial adenomatous polyposis (FAP) and Gardner′s syndrome, both of which lead to the development of numerous adenomatous polyps that can progress to colon cancer. APC protein, consisting of 2,843 amino acids, is predominantly localized at the cell membrane, where APC interacts with key structural components of intracellular junctions. Notably, APC competes with E-cadherin for binding to β-catenin and γ-catenin, which are essential for cellular adhesion. The dynamic interactions between APC, E-cadherin, and the catenins are further regulated by α-catenin, linking the cytoskeleton to the cell membrane and ensuring proper cellular architecture. This intricate network underscores the importance of APC in cellular signaling pathways and APC′s role as a tumor suppressor, highlighting the significance of APC monoclonal antibody (ALi 12-28) in cancer research and diagnostics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-APC Antibody (ALi 12-28) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-APC Antibody (ALi 12-28) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

APC Antibody (ALi 12-28) Specifications

Alternate Product Name: APC

Product Clone Name: ALi 12-28

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-433

Epitope Target: APC

Epitope Species: human

Product Reactivity/Detection: APC

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for APC Antibody (ALi 12-28): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred APC antibody is APC Antibody (F-3): sc-9998.