APC Antibody (F-3): sc-9998

APC Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects adenomatous polyposis coli (APC) protein of mouse, rat, and human origin through various applications including western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and enzyme-linked immunosorbent assay. APC monoclonal antibody (F-3) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. APC protein plays a crucial role in regulating cell adhesion and maintaining the integrity of epithelial tissues, which is vital for preventing tumorigenesis. Mutations in APC gene are commonly associated with familial adenomatous polyposis and Gardner′s syndrome, conditions characterized by the development of numerous adenomatous polyps that can lead to colon cancer. APC protein, consisting of 2,843 amino acids, is ubiquitously expressed and interacts with key structural components of intracellular junctions, including β-catenin and γ-catenin, which are essential for cellular adhesion. APC competes with E-cadherin for binding to specific regions of β- and γ-catenin, and interactions between cytoskeleton and APC-E-cadherin-β/γ-catenin complex are mediated by α-catenin, highlighting APC′s importance in cellular signaling pathways that regulate cell growth and differentiation.

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APC Antibody (F-3) References:

1. The APC gene in colorectal cancer.  |  Fodde, R. 2002. Eur J Cancer. 38: 867-71. PMID: 11978510
2. Mutations of chromosome 5q21 genes in FAP and colorectal cancer patients.  |  Nishisho, I., et al. 1991. Science. 253: 665-9. PMID: 1651563
3. APC and oncogenic KRAS are synergistic in enhancing Wnt signaling in intestinal tumor formation and progression.  |  Janssen, KP., et al. 2006. Gastroenterology. 131: 1096-109. PMID: 17030180
4. Myc deletion rescues Apc deficiency in the small intestine.  |  Sansom, OJ., et al. 2007. Nature. 446: 676-9. PMID: 17377531
5. Germ-line and somatic mutations of the APC gene and/or ss catenin gene in the occurrence of FAP associated thyroid carcinoma.  |  Cetta, F., et al. 2007. World J Surg. 31: 1366-7; author reply 1368-9. PMID: 17426899
6. B-catenin deficiency, but not Myc deletion, suppresses the immediate phenotypes of APC loss in the liver.  |  Reed, KR., et al. 2008. Proc Natl Acad Sci U S A. 105: 18919-23. PMID: 19033191
7. Mechanical factors activate beta-catenin-dependent oncogene expression in APC mouse colon.  |  Whitehead, J., et al. 2008. HFSP J. 2: 286-94. PMID: 19404440
8. Apc inhibition of Wnt signaling regulates supernumerary tooth formation during embryogenesis and throughout adulthood.  |  Wang, XP., et al. 2009. Development. 136: 1939-49. PMID: 19429790
9. The APC/C and CK1 in the developing brain.  |  Penas, C., et al. 2015. Oncotarget. 6: 16792-3. PMID: 26219466
10. APC Promoter Methylation in Gastrointestinal Cancer.  |  Zhu, L., et al. 2021. Front Oncol. 11: 653222. PMID: 33968756
11. METTL3 promotes tumour development by decreasing APC expression mediated by APC mRNA N6-methyladenosine-dependent YTHDF binding.  |  Wang, W., et al. 2021. Nat Commun. 12: 3803. PMID: 34155197
12. High resolution genetic map of the adenomatous polyposis coli gene (APC) region.  |  Olschwang, S., et al. 1995. Am J Med Genet. 56: 413-9. PMID: 7604852

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-APC Antibody (F-3) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-APC Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

APC Antibody (F-3) Specifications

Alternate Product Name: APC

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-289

Epitope Target: APC

Epitope Species: human

Product Reactivity/Detection: APC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for APC Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin