AP-180 Antibody (AP180-I): sc-58229

AP-180 Antibody (AP180-I) is a mouse monoclonal IgG2b kappa light chain antibody that detects AP-180 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-AP-180 antibody (AP180-I) is available as a non-conjugated format, specifically designed to facilitate the study of this critical protein involved in synaptic vesicle trafficking. AP-180, also known as SNAP91 or CALM, is a 907 amino acid cell membrane protein that plays a vital role in the assembly of clathrin-coated pits and vesicles, which are essential for receptor-mediated endocytosis. AP-180 contains an epsin N-terminal homology (ENTH) domain that allows binding to clathrin triskelia through its N-terminal clathrin binding site, promoting the formation of 60-70 nm coats necessary for vesicle transport. This process is crucial for maintaining cellular homeostasis and facilitating neurotransmitter release in neurons. The gene encoding AP-180 is located on human chromosome 6q14.2 and mouse chromosome 9 E3.1, and exists in three alternatively spliced isoforms, highlighting its functional diversity. AP-180 monoclonal antibody (AP180-I) enables researchers to gain insights into the mechanisms of synaptic transmission and the role of clathrin-mediated endocytosis in various physiological and pathological processes.

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AP-180 Antibody (AP180-I) References:

1. Conservation and diversity in families of coated vesicle adaptins.  |  Ponnambalam, S., et al. 1990. J Biol Chem. 265: 4814-20. PMID: 1969413
2. Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus.  |  Robinson, MS. 1990. J Cell Biol. 111: 2319-26. PMID: 2126014
3. Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2.  |  Kirchhausen, T., et al. 1989. Proc Natl Acad Sci U S A. 86: 2612-6. PMID: 2495531
4. Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins).  |  Robinson, MS. 1989. J Cell Biol. 108: 833-42. PMID: 2564002
5. Expression and localization of alpha-adaptin isoforms.  |  Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
6. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site.  |  Morris, SA., et al. 1993. EMBO J. 12: 667-75. PMID: 8440257
7. The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.  |  Dreyling, MH., et al. 1996. Proc Natl Acad Sci U S A. 93: 4804-9. PMID: 8643484
8. Endocytosis and molecular sorting.  |  Mellman, I. 1996. Annu Rev Cell Dev Biol. 12: 575-625. PMID: 8970738

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AP-180 Antibody (AP180-I) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-AP-180 Antibody (AP180-I) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AP-180 Antibody (AP180-I) Specifications

Alternate Product Name: AP-180

Product Clone Name: AP180-I

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: AP-180

Epitope Species: bovine

Product Reactivity/Detection: AP-180

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for AP-180 Antibody (AP180-I): N/A