Anti-Annexin VI Antibody (G-10) recommended for detection of Annexin VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Annexin VI Antibody (G-10): sc-166807

Anti-Annexin VI Antibody (G-10) is rated 5.0 out of 5 by 4.
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Datasheets

    • Annexin VI Antibody (G-10) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 497-610 of Annexin VI of human origin
    • recommended for detection of Annexin VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for Annexin VI Antibody (G-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Annexin VI Antibody (G-10) (see ordering information below).
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I am using Annexin VI (G-10): sc-166807 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Publishable data obtained by WB using human Publishable data obtained by WB using human lymph and plasma samples. -SCBT Publication Review
Date published: 2015-04-24
Rated 5 out of 5 by from Positive detection of endogenous Annexin Positive detection of endogenous Annexin VI by WB using HeLa, L8, JAR, JEG-3 and MES-SA/Dx5 whole cell lysates. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin VI protein via Western Blot analysis. -SCBT QC
Date published: 2015-01-09
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